Monitoring Calcium Oscillations in Fertilized Mouse Eggs - Université de Rennes Access content directly
Journal Articles Methods in Molecular Biology Year : 2016

Monitoring Calcium Oscillations in Fertilized Mouse Eggs

Abstract

In mammalian species, including human, fertilization is characterized by the triggering of long-lasting calcium (Ca(2+)) oscillations in the egg cytoplasm. The monitoring of these Ca(2+) oscillations is a valuable technique to demonstrate that fertilization has occurred, to study egg activation events elicited downstream of the Ca(2+) signal, as well as to evaluate sperm quality. This chapter describes our protocol to monitor sperm-induced Ca(2+) oscillations in mouse eggs, using fluorescence microscopy techniques and the Fura-2-AM ratiometric Ca(2+) indicator.
Fichier principal
Vignette du fichier
Monitoring Ca2+ oscillations in fertilized mouse eggs - G.Halet.pdf (299.4 Ko) Télécharger le fichier
Origin Files produced by the author(s)
Loading...

Dates and versions

hal-01439381 , version 1 (24-03-2017)

Identifiers

Cite

Guillaume Halet. Monitoring Calcium Oscillations in Fertilized Mouse Eggs. Methods in Molecular Biology, 2016, 1457, pp.231-240. ⟨10.1007/978-1-4939-3795-0_17⟩. ⟨hal-01439381⟩
172 View
211 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More