%0 Journal Article %T Monitoring Calcium Oscillations in Fertilized Mouse Eggs %+ Institut de Génétique et Développement de Rennes (IGDR) %A Halet, Guillaume %Z My laboratory is supported by the CNRS, the University of Rennes 1, Agence de la biomédecine and Ligue contre le cancer. %< avec comité de lecture %@ 1064-3745 %J Methods in Molecular Biology %I Humana Press/Springer Imprint %V 1457 %P 231-240 %8 2016 %D 2016 %R 10.1007/978-1-4939-3795-0_17 %M 27557585 %K Oocyte %K Egg %K Sperm %K Fertilization %K Calcium %K Ca2+ %K Fluorescence %K Imaging %Z Life Sciences [q-bio] %Z Life Sciences [q-bio]/GeneticsJournal articles %X In mammalian species, including human, fertilization is characterized by the triggering of long-lasting calcium (Ca(2+)) oscillations in the egg cytoplasm. The monitoring of these Ca(2+) oscillations is a valuable technique to demonstrate that fertilization has occurred, to study egg activation events elicited downstream of the Ca(2+) signal, as well as to evaluate sperm quality. This chapter describes our protocol to monitor sperm-induced Ca(2+) oscillations in mouse eggs, using fluorescence microscopy techniques and the Fura-2-AM ratiometric Ca(2+) indicator. %G English %2 https://univ-rennes.hal.science/hal-01439381/document %2 https://univ-rennes.hal.science/hal-01439381/file/Monitoring%20Ca2%2B%20oscillations%20in%20fertilized%20mouse%20eggs%20-%20G.Halet.pdf %L hal-01439381 %U https://univ-rennes.hal.science/hal-01439381 %~ UNIV-RENNES1 %~ CNRS %~ IFR140 %~ IGDR %~ STATS-UR1 %~ IGDR-DO %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES