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Poster De Conférence Année : 2022

Impact of repeated exposure to indoor dust on human macrophage polarization profile

Résumé

As macrophages are essential in immune response to environmental particles, this study evaluates the effects of repeated exposure to PM2.5 fraction of indoor particles (Organic contaminants in house dust, SRM2585) towards macrophage activation. Monocyte-derived macrophages (MDM) were exposed four times to PM2.5 fraction at 1 and 10 μg/cm2. Cell viability, phenotype and secretory capacities were evaluated after the last day of exposure (day 4) and post-exposure (day 11). Exposure to 1 and 10 μg/ml of PM2.5 favored the expression of some pro-inflammatory markers (CD40 and CD80) and a down-expression of pro-fibrotic markers (CD163 and CD204) in MDM at day 4. Such effect were reversible at day 11 for the low but not the high concentration of PM2.5. Moreover, MDM exposed to 10 μg/cm2 PM2.5 secreted pro-inflammatory molecules such as IL-6, SERPINE1 and CCL-chemokines. A repeated exposure of human macrophages to PM2.5 confers a reversible pro-inflammatory phenotype depending on the concentration.

Domaines

Toxicologie
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Dates et versions

hal-04066882 , version 1 (12-04-2023)

Identifiants

  • HAL Id : hal-04066882 , version 1

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Marie Lelong, Carine El Hajjar, Ivannah Pottier, Veronique Andre, Valérie Lecureur. Impact of repeated exposure to indoor dust on human macrophage polarization profile. Indoor Air 2022 conference, 17th International Conference on Indoor Air Quality and Climate, Jun 2022, Kupio, Finland. ⟨hal-04066882⟩
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