Setup and use of HepaRG cells in cholestasis research - Université de Rennes Access content directly
Book Sections Year : 2019

Setup and use of HepaRG cells in cholestasis research


Since HepaRG cells can differentiate into well-polarized mature hepatocyte-like cells that synthesize, conjugate, and secrete bile acids, they represent an appropriate surrogate to primary human hepatocytes for investigations on drug-induced cholestasis mechanisms. In this chapter, culture conditions for obtaining HepaRG hepatocytes and the main methods used to detect cholestatic potential of drugs are described. Assays for evaluation of bile canaliculi dynamics and morphology are mainly based on time-lapse and phase-contrast microscopy analysis. Bile acid uptake, trafficking, and efflux are investigated using fluorescent probes. Individual bile acids are quantified in both culture media and cell layers by high-pressure liquid chromatography/tandem mass spectrometry. Preferential cellular accumulation of toxic hydrophobic bile acids is easily evidenced when exogenous primary and secondary bile acids are added to the culture medium. © Springer Science+Business Media, LLC, part of Springer Nature 2019.
No file

Dates and versions

hal-02161939 , version 1 (21-06-2019)



C. Guguen-Guillouzo, A. Guillouzo. Setup and use of HepaRG cells in cholestasis research. Experimental Cholestasis Research, 1981, Humana Press Inc., pp.291-312, 2019, ⟨10.1007/978-1-4939-9420-5_19⟩. ⟨hal-02161939⟩
14 View
0 Download



Gmail Facebook X LinkedIn More