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Article Dans Une Revue Life Science Alliance Année : 2018

CHRAC/ACF contribute to the repressive ground state of chromatin

Alessandro Scacchetti
  • Fonction : Auteur
Laura Brueckner
  • Fonction : Auteur
Dhawal Jain
  • Fonction : Auteur
Bas van Steensel
  • Fonction : Auteur
Tobias Straub
  • Fonction : Auteur
Peter Becker
  • Fonction : Auteur correspondant

Résumé

The chromatin remodeling complexes chromatin accessibility complex and ATP-utilizing chromatin assembly and remodeling factor (ACF) combine the ATPase ISWI with the signature subunit ACF1. These enzymes catalyze well-studied nucleo-some sliding reactions in vitro, but how their actions affect physiological gene expression remains unclear. Here, we explored the influence of Drosophila melanogaster chromatin accessibility complex/ACF on transcription by using complementary gain-and loss-of-function approaches. Targeting ACF1 to multiple reporter genes inserted at many different genomic locations revealed a context-dependent inactivation of poorly transcribed reporters in repressive chromatin. Accordingly , single-embryo transcriptome analysis of an Acf knockout allele showed that only lowly expressed genes are derepressed in the absence of ACF1. Finally, the nucleosome arrays in Acf-deficient chromatin show loss of physiological regularity, particularly in transcriptionally inactive domains. Taken together, our results highlight that ACF1-containing remodeling factors contribute to the establishment of an inactive ground state of the genome through chromatin organization.
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hal-02109020 , version 1 (24-04-2019)

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Alessandro Scacchetti, Laura Brueckner, Dhawal Jain, Tamás Schauer, Xu Zhang, et al.. CHRAC/ACF contribute to the repressive ground state of chromatin. Life Science Alliance, 2018, 1 (1), pp.e201800024. ⟨10.26508/lsa.201800024⟩. ⟨hal-02109020⟩
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