Gene Introgression in Weeds Depends on Initial Gene Location in the Crop: Brassica napus - Raphanus raphanistrum Model - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Genetics Année : 2017

Gene Introgression in Weeds Depends on Initial Gene Location in the Crop: Brassica napus - Raphanus raphanistrum Model

Gwénaëlle Thomas
  • Fonction : Auteur
  • PersonId : 1019665
Sylvie Negre
  • Fonction : Auteur
  • PersonId : 1204103
Bachar Alrustom
  • Fonction : Auteur
  • PersonId : 1019007
Eric Jenczewski

Résumé

The effect of gene location within a crop genome on its transfer to a weed genome remains an open question for gene flow assessment. To elucidate this question, we analyzed advanced generations of intergeneric hybrids, derived from an initial pollination of known oilseed rape varieties (Brassica napus, AACC, 2n=38) by a local population of wild radish (Raphanus raphanistrum, RrRr, 2n=18). After five generations of recurrent pollination, 307 G5 plants with a chromosome number similar to wild radish were genotyped using 105 B. napus specific markers well distributed along the chromosomes. They revealed that 49.8% of G5 plants carried at least one B. napus genomic region. According to the frequency of B. napus markers (0 to 28%), four classes were defined: Class 1 (near zero frequency) with 75 markers covering ~70% of oilseed rape genome, Class 2 (low frequency) with 20 markers located on 11 genomic regions, Class 3 (high frequency) with 8 markers on 3 genomic regions and Class 4 (higher frequency) with 2 adjacent markers detected on A10. Therefore, some regions of the oilseed rape genome are more prone than others to be introgressed into wild radish. Inheritance and growth of plant progeny revealed that genomic regions of oilseed rape could be stably introduced into wild radish and variably impact the plant fitness (plant height and seed number). Our results pinpoint that novel technologies enabling the targeted insertion of transgenes should select genomic regions that are less likely to be introgressed into the weed genome, thereby reducing gene flow.

Dates et versions

hal-01607924 , version 1 (03-10-2017)

Licence

Paternité - Partage selon les Conditions Initiales

Identifiants

Citer

Katarzyna Adamczyk, Sabrina Delaunay, Anne Vannier, Caroline François, Gwénaëlle Thomas, et al.. Gene Introgression in Weeds Depends on Initial Gene Location in the Crop: Brassica napus - Raphanus raphanistrum Model. Genetics, 2017, 206 (3), pp.1361-1372. ⟨10.1534/genetics.117.201715⟩. ⟨hal-01607924⟩
184 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More