The IGH locus relocalizes to a "recombination compartment" in the perinucleolar region of differentiating B-lymphocytes - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Oncotarget Année : 2017

The IGH locus relocalizes to a "recombination compartment" in the perinucleolar region of differentiating B-lymphocytes

Aleksandr Barinov
Evgeny Ivashkin
  • Fonction : Auteur

Résumé

The immunoglobulin heavy chain (IGH) gene loci are subject to specific recombination events during B-cell differentiation including somatic hypermutation and class switch recombination which mark the end of immunoglobulin gene maturation in germinal centers of secondary lymph nodes. These two events rely on the activity of activation-induced cytidine deaminase (AID) which requires DNA double strand breaks be created, a potential danger to the cell. Applying 3D-fluorescence in situ hybridization coupled with immunofluorescence staining to a previously described experimental system recapitulating normal B-cell differentiation ex vivo, we have kinetically analyzed the radial positioning of the two IGH gene loci as well as their proximity with the nucleolus, heterochromatin and γH2AX foci. Our observations are consistent with the proposal that these IGH gene rearrangements take place in a specific perinucleolar "recombination compartment" where AID could be sequestered thus limiting the extent of its potentially deleterious off-target effects.

Domaines

Cancer Immunologie
Fichier principal
Vignette du fichier
The IGH locus relocalizes.pdf (6.58 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01560456 , version 1 (03-05-2018)

Identifiants

Citer

Andrey Pichugin, Olga V. Iarovaia, Alexey Gavrilov, Ilya Sklyar, Natalja Barinova, et al.. The IGH locus relocalizes to a "recombination compartment" in the perinucleolar region of differentiating B-lymphocytes. Oncotarget, 2017, 8 (25), pp.40079-40089. ⟨10.18632/oncotarget.16941⟩. ⟨hal-01560456⟩
106 Consultations
68 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More