Ibuprofen results in alterations of human fetal testis development - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2017

Ibuprofen results in alterations of human fetal testis development

Résumé

Among pregnant women ibuprofen is one of the most frequently used pharmaceutical compounds with up to 28% reporting use. Regardless of this, it remains unknown whether ibuprofen could act as an endocrine disruptor as reported for fellow analgesics paracetamol and aspirin. To investigate this, we exposed human fetal testes (7-17 gestational weeks (GW)) to ibuprofen using ex vivo culture and xenograft systems. Ibuprofen suppressed testosterone and Leydig cell hormone INSL3 during culture of 8-9 GW fetal testes with concomitant reduction in expression of the steroidogenic enzymes CYP11A1, CYP17A1 and HSD17B3, and of INSL3. Testosterone was not suppressed in testes from fetuses younger than 8 GW, older than 10-12 GW, or in second trimester xenografted testes (14-17 GW). Ex vivo, ibuprofen also affected Sertoli cell by suppressing AMH production and mRNA expression of AMH, SOX9, DHH, and COL2A1. While PGE2 production was suppressed by ibuprofen, PGD2 production was not. Germ cell transcripts POU5F1, TFAP2C, LIN28A, ALPP and KIT were also reduced by ibuprofen. We conclude that, at concentrations relevant to human exposure and within a particular narrow 'early window' of sensitivity within first trimester, ibuprofen causes direct endocrine disturbances in the human fetal testis and alteration of the germ cell biology.
Fichier principal
Vignette du fichier
Maamar_Ibuprofen results in alterations.pdf (2.19 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01517095 , version 1 (28-06-2018)

Licence

Paternité

Identifiants

Citer

M. Ben Maamar, Lauriane Lesné, K. Hennig, C. Desdoits-Lethimonier, K.R. Kilcoyne, et al.. Ibuprofen results in alterations of human fetal testis development. Scientific Reports, 2017, 7 (1), pp.44184. ⟨10.1038/srep44184⟩. ⟨hal-01517095⟩
305 Consultations
142 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More