Emergence of long-lived autoreactive plasma cells in the spleen of primary warm auto-immune hemolytic anemia patients treated with rituximab - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Journal of Autoimmunity Année : 2015

Emergence of long-lived autoreactive plasma cells in the spleen of primary warm auto-immune hemolytic anemia patients treated with rituximab

Benoît Vingert
  • Fonction : Auteur
Julien Moroch
  • Fonction : Auteur
Julie Ripa
  • Fonction : Auteur
Karin Tarte
Jérôme Mégret
  • Fonction : Auteur
Simon Le Gallou
  • Fonction : Auteur
Lan Thai
  • Fonction : Auteur
Bernard Bonnotte
Bertrand Godeau
  • Fonction : Auteur
  • PersonId : 882148
Jean-Claude Weill
  • Fonction : Auteur
  • PersonId : 851048
Claude-Agnès Reynaud
  • Fonction : Auteur
  • PersonId : 851049

Résumé

Primary warm autoimmune hemolytic anemia (wAIHA) is a rare autoimmune disease in which red blood cells are eliminated by IgG autoantibodies. We analyzed the antibody-secreting cells in the spleen and the peripheral blood of wAIHA patients in various contexts of treatment. Plasmablasts were observed in peripheral blood of newly diagnosed wAIHA patients and, accordingly, active germinal center reactions were present in the spleen of patients receiving short-term corticosteroid therapy. Long-term corticosteroid regimens markedly reduced this response while splenic plasma cells were able to persist, a fraction of them secreting anti-red blood cell IgG in vitro. In wAIHA patients treated by rituximab and who underwent splenectomy because of treatment failure, plasma cells were still present in the spleen, some of them being autoreactive. By using a set of diagnostic genes that allowed us to assess the plasma cell maturation stage, we observed that these cells displayed a long-lived program, differing from the one of plasma cells from healthy donors or from wAIHA patients with various immunosuppressant treatments, and more similar to the one of normal long-lived bone-marrow plasma cells. Interestingly, an increased level of B-cell activating factor (BAFF) was observed in the supernatant of spleen cell cultures from such rituximab-treated wAIHA patients. These results suggest, in line with our previous report on primary immune thrombocytopenia, that the B-cell depletion induced by rituximab promoted a suitable environment for the maturation and survival of auto-immune long-lived plasma cells in the spleen
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Dates et versions

hal-01169799 , version 1 (30-06-2015)

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Citer

Matthieu Mahevas, Marc Michel, Benoît Vingert, Julien Moroch, David Boutboul, et al.. Emergence of long-lived autoreactive plasma cells in the spleen of primary warm auto-immune hemolytic anemia patients treated with rituximab. Journal of Autoimmunity, 2015, 62, pp.22-30. ⟨10.1016/j.jaut.2015.05.006⟩. ⟨hal-01169799⟩
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