Diversity and comparative genomics of Microviridae in Sphagnum-dominated peatlands - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Frontiers in Microbiology Année : 2015

Diversity and comparative genomics of Microviridae in Sphagnum-dominated peatlands

Résumé

Microviridae, a family of bacteria-infecting ssDNA viruses, is one of the still poorly characterized bacteriophage groups, event hough it includes phage PhiX174, one of the main models in virology for genomic and capsid structure studies. Recent studies suggest that they are diverse and well represented in marine and freshwater virioplankton as well as in human microbiomes. However,theirdiversity,abundance,andecologicalrolearecompletelyunknowninsoilecosystems.Herewepresentthecomparativeanalysisof17completelyassembledMicroviridaegenomesfrom12viromesofaSphagnum-dominatedpeatland.PhylogeneticanalysisoftheconservedmajorcapsidproteinsequencesrevealedtheaffiliationtoGokushovirinaeandPichovirinaeaswellastotwonewlydefinedsubfamilies,theAravirinaeandStokavirinae.Additionally,twonewdistinctprophageswereidentifiedinthegenomesofParabacteroidesmerdaeandParabacteroidesdistasonisrepresentingapotentialnewsubfamilyofMicroviridae.Thedifferentiationofthesubfamilieswasconfirmedbygeneorderandsimilarityanalysis.Relativeabundanceanalysisusingtheaffiliationofthemajorcapsidprotein(VP1)revealedthatGokushovirinae,followedbyAravirinae,arethemostabundantMicroviridaein11outof12peatviromes.SequencesmatchingtheGokushovirinaeandAravirinaeVP1matchingsequences,respectively,accountedforupto4.19and0.65%ofthetotalnumberofsequencesinthecorrespondingvirome,respectively.InthisstudyweprovidenewgenomeinformationofMicroviridaeandpavethewaytowardquantitativeestimationsofMicroviridaesubfamilies.
Fichier principal
Vignette du fichier
fmicb-06-00375.pdf (6.63 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01149546 , version 1 (10-07-2022)

Licence

Paternité

Identifiants

Citer

Achim Quaiser, Alexis Dufresne, Flore Ballaud, Simon Roux, Yvan Zivanovic, et al.. Diversity and comparative genomics of Microviridae in Sphagnum-dominated peatlands. Frontiers in Microbiology, 2015, 6, pp.375. ⟨10.3389/fmicb.2015.00375⟩. ⟨hal-01149546⟩
390 Consultations
23 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More