EFFECTS OF A NOVEL CERAMIC BIOMATERIAL ON IMMUNE MODULATORY PROPERTIES AND DIFFERENTIATION POTENTIAL OF MESENCHYMAL STROMAL CELLS - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Cytotherapy Année : 2014

EFFECTS OF A NOVEL CERAMIC BIOMATERIAL ON IMMUNE MODULATORY PROPERTIES AND DIFFERENTIATION POTENTIAL OF MESENCHYMAL STROMAL CELLS

Fabien Guilloton
  • Fonction : Auteur
Luc Sensebé
  • Fonction : Auteur
  • PersonId : 940670
Serge Baroth
  • Fonction : Auteur
Rosaria Giordano
  • Fonction : Auteur
Cristiana Lavazza
  • Fonction : Auteur
Lorenza Lazzari
  • Fonction : Auteur
Massimo Dominici
  • Fonction : Auteur
Karin Tarte
  • Fonction : Auteur
  • PersonId : 893199
Mauro Krampera
  • Fonction : Auteur
  • PersonId : 945917

Résumé

The aim of this study was to assess the immune modulatory properties of human mesenchymal stromal cells obtained from bone marrow (BM-MSCs), fat (ASCs) and cord blood (CB-MSCs) in the presence of a novel hydroxyapatite and tricalcium-phosphate (HA/TCP) biomaterial as scaffold for MSC delivery. In resting conditions, a short-term culture with HA/TCP did not modulate the anti-apoptotic and suppressive features of the various MSC types towards T, B and NK cells; in addition, when primed with inflammatory cytokines, MSC maintained or not on HA/TCP similarlyincreased their suppressive capacities. The long-term culture of BM-MSCs with HA/TCP induced an osteoblast-like phenotype with up-regulation of OSTERIX and OSTEOCALCIN, similarly to what obtained with dexamethasone and, to a higher extent, BMP-4 treatment. MSC-derived osteoblasts did not trigger immune cell activation, but were less efficient than undifferentiated MSCs in inhibiting stimulated T and NK cells. Interestingly, their suppressive machinery included not only the activation of IDO, which plays a central role in T-cell inhibition, but also COX-2 that was not significantly involved in immune modulatory effect of human undifferentiated MSCs. COX-2 is significantly involved in bone healing, suggesting that its induction by HA/TCP could also contribute to the therapeutic activity of MSC for bone tissue engineering.
Fichier non déposé

Dates et versions

hal-00988244 , version 1 (07-05-2014)

Identifiants

Citer

Giulio Bassi, Fabien Guilloton, Cédric Ménard, Mariano Di Trapani, Luciano Pacelli, et al.. EFFECTS OF A NOVEL CERAMIC BIOMATERIAL ON IMMUNE MODULATORY PROPERTIES AND DIFFERENTIATION POTENTIAL OF MESENCHYMAL STROMAL CELLS. Cytotherapy, 2014, 16 (4), pp.S90-S91. ⟨10.1016/j.jcyt.2014.01.336⟩. ⟨hal-00988244⟩
168 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More