An atlas of chromatoid body components. - Université de Rennes Access content directly
Journal Articles RNA Year : 2014

An atlas of chromatoid body components.

Oliver Meikar
  • Function : Author
Vasily V Vagin
  • Function : Author
Karin Sõstar
  • Function : Author
Aurélie Lardenois
  • Function : Author
Molly Hammell
  • Function : Author
Ying Jin
  • Function : Author
Matteo da Ros
  • Function : Author
Kaja A Wasik
  • Function : Author
Jorma Toppari
  • Function : Author
Gregory J Hannon
  • Function : Author
Noora Kotaja
  • Function : Correspondent author
  • PersonId : 955809

Connectez-vous pour contacter l'auteur


The genome of male germ cells is actively transcribed during spermatogenesis to produce phase-specific protein-coding mRNAs and a considerable amount of different noncoding RNAs. Ribonucleoprotein (RNP) granule-mediated RNA regulation provides a powerful means to secure the quality and correct expression of the requisite transcripts. Haploid spermatids are characterized by a unique, unusually large cytoplasmic granule, the chromatoid body (CB), which emerges during the switch between the meiotic and post-meiotic phases of spermatogenesis. To better understand the role of the CB in male germ cell differentiation, we isolated CBs from mouse testes and revealed its full RNA and protein composition. We showed that the CB is mainly composed of RNA-binding proteins and other proteins involved RNA regulation. The CB was loaded with RNA, including pachytene piRNAs, a diverse set of mRNAs, and a number of uncharacterized long noncoding transcripts. The CB was demonstrated to accumulate nascent RNA during all the steps of round spermatid differentiation. Our results revealed the CB as a large germ cell-specific RNP platform that is involved in the control of the highly complex transcriptome of haploid male germ cells.

Dates and versions

hal-00985894 , version 1 (30-04-2014)



Oliver Meikar, Vasily V Vagin, Frédéric Chalmel, Karin Sõstar, Aurélie Lardenois, et al.. An atlas of chromatoid body components.. RNA, 2014, 20 (4), pp.483-95. ⟨10.1261/rna.043729.113⟩. ⟨hal-00985894⟩
91 View
0 Download



Gmail Facebook Twitter LinkedIn More