Inhibition of human mesenchymal stem cell-derived adipogenesis by the environmental contaminant benzo(a)pyrene. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Toxicology in Vitro Année : 2009

Inhibition of human mesenchymal stem cell-derived adipogenesis by the environmental contaminant benzo(a)pyrene.

Résumé

Polycyclic aromatic hydrocarbons (PAHs) such as benzo(a)pyrene (BP) are environmental contaminants exerting various toxic effects. PAHs have notably been found to inhibit adipogenesis in rodent species. To determine whether a similar process concerns human cells, we have analyzed the effects of BP towards differentiation of human cultured mesenchymal stem cells (MSC) into adipocytes, triggered by a pro-adipogenic culture medium. BP was found to markedly prevent formation of lipid vesicles, cellular lipid accumulation and up-regulation of adipogenic markers such as fatty acid binding protein-4 and glyceraldehyde-3-phosphate dehydrogenase, which represent major hallmarks of human MSC-derived adipocytes. The aryl hydrocarbon receptor (AhR), known to mediate most of the toxic effects of PAHs, was demonstrated to be present and functional in human MSC. 2,3,7,8-tetrachlorodibenzo-p-dioxin, an AhR agonist like BP, was found to inhibit lipid accumulation in human MSC cultured with adipogenic medium, in contrast to the PAH benzo(e)pyrene, known to not, or only poorly, interact with AhR. Moreover, BP inhibitory effect toward lipid accumulation in MSC exposed to adipogenic medium was fully counteracted by co-treatment with the AhR antagonist alpha-naphtoflavone. Taken together, these data indicate that environmental PAHs like BP can likely inhibit human adipogenesis in an AhR-dependent manner.

Dates et versions

hal-00673359 , version 1 (23-02-2012)

Identifiants

Citer

Normand Podechard, Olivier Fardel, Michel Corolleur, Marc Bernard, Valérie Lecureur. Inhibition of human mesenchymal stem cell-derived adipogenesis by the environmental contaminant benzo(a)pyrene.. Toxicology in Vitro, 2009, 23 (6), pp.1139-44. ⟨10.1016/j.tiv.2009.05.011⟩. ⟨hal-00673359⟩
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