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Article Dans Une Revue Molecular Therapy Année : 2005

A ubiquitin-based assay for the cytosolic uptake of protein transduction domains.

Résumé

Protein transduction domains (PTDs) are promising tools for transducing presynthesized polypeptides across the plasma membrane. However, the development and optimization of PTDs are hampered by many technical problems and artifacts resulting notably from the tight binding of PTDs to the cell surface and the difficulty in discriminating, through imagery analyses, truly cytosolic from cytoplasmic vesicular compartments. To circumvent these problems, we have developed an unambiguous enzymatic assay of the cytosolic uptake of PTD-driven proteins, based on the processing by ubiquitin-specific C-terminal proteases (DUBs). This method, coupled with fluorometry and fluorescence microscopy, shows that the TAT PTD derived from human immunodeficiency virus type 1 is rapidly taken up by cells but fails to reach their cytosol, except when dendritic cells, which are known to take up circulating antigens for cross-presentation, are used. In addition to its usefulness in assessing cytosolic uptake, DUB processing of PTD-linked proteins can ensure the intracellular release of cargo proteins, which might prove helpful for MHC-I-based vaccination or intracellular delivery of biologically active polypeptides.

Dates et versions

hal-00293600 , version 1 (07-07-2008)

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Fabien Loison, Philippe Nizard, Tony Sourisseau, Pascale Le Goff, Laure Debure, et al.. A ubiquitin-based assay for the cytosolic uptake of protein transduction domains.. Molecular Therapy, 2005, 11 (2), pp.205-14. ⟨10.1016/j.ymthe.2004.10.010⟩. ⟨hal-00293600⟩
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