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Chapitre D'ouvrage Année : 2014

Modern Tools to Study Nuclear Pore Complexes and Nucleocytoplasmic Transport in Caenorhabditis elegans

Peter Askjaer
  • Fonction : Auteur
Vincent Galy
Peter Meister
  • Fonction : Auteur

Résumé

The nematode Caenorhabditis elegans is characterized by many features that make it highly attractive to study nuclear pore complexes (NPCs) and nucleocytoplasmic transport. NPC composition and structure are highly conserved in nematodes and being amenable to a variety of genetic manipulations, key aspects of nuclear envelope dynamics can be observed in great details during breakdown, reassembly, and interphase. In this chapter, we provide an overview of some of the most relevant modern techniques that allow researchers unfamiliar with C. elegans to embark on studies of nucleoporins in an intact organism through its development from zygote to aging adult. We focus on methods relevant to generate loss-of-function phenotypes and their analysis by advanced microscopy. Extensive references to available reagents, such as mutants, transgenic strains, and antibodies are equally useful to scientists with or without prior C. elegans or nucleoporin experience.
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Dates et versions

hal-01543607 , version 1 (21-06-2017)

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Citer

Peter Askjaer, Vincent Galy, Peter Meister. Modern Tools to Study Nuclear Pore Complexes and Nucleocytoplasmic Transport in Caenorhabditis elegans. Doye, V. NUCLEAR PORE COMPLEXES AND NUCLEOCYTOPLASMIC TRANSPORT - METHODS, 122, ELSEVIER ACADEMIC PRESS INC, pp.277-310, 2014, Methods in Cell Biology, 978-0-12-417160-2. ⟨10.1016/B978-0-12-417160-2.00013-8⟩. ⟨hal-01543607⟩
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