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Journal Articles Journal of Cell Science Year : 2016

Sharing of mitotic pre-ribosomal particles between daughter cells.


Ribosome biogenesis is a fundamental multistep process initiated by the synthesis of 90S pre-ribosomal particles in the nucleoli of higher eukaryotes. Even though synthesis of ribosomes stops during mitosis while nucleoli disappear, mitotic pre-ribosomal particles persist as observed in pre-nucleolar bodies (PNBs) during telophase. To further understand the relationship between the nucleolus and the PNBs, the presence and the fate of the mitotic pre-ribosomal particles during cell division were investigated. We demonstrate that the recently synthesized 45S precursor ribosomal RNAs (pre-rRNAs) as well as the 32S and 30S pre-rRNAs are maintained during mitosis and associated with the chromosome periphery together with pre-rRNA processing factors. Maturation of the mitotic pre-ribosomal particles, as assessed by the stability of the mitotic pre-rRNAs, is transiently arrested during mitosis by a cyclin-dependent kinase (CDK)1-cyclin-B-dependent mechanism and can be restored by CDK inhibitor treatments. At the M-G1 transition, the resumption of mitotic pre-rRNA processing in PNBs does not induce the disappearance of PNBs; this only occurs when functional nucleoli reform. Strikingly, during their maturation process, mitotic pre-rRNAs localize in reforming nucleoli.
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Dates and versions

hal-01323855 , version 1 (30-01-2017)



Valentina Sirri, Nathalie Jourdan, Danièle Hernandez-Verdun, Pascal Roussel. Sharing of mitotic pre-ribosomal particles between daughter cells.. Journal of Cell Science, 2016, 129 (8), pp.1592-1604. ⟨10.1242/jcs.180521⟩. ⟨hal-01323855⟩
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