Demultiplexing Ig repertoires by parallel mRNA/DNA sequencing shows major differential alterations in severe COVID-19 - Université de Rennes Accéder directement au contenu
Article Dans Une Revue iScience Année : 2023

Demultiplexing Ig repertoires by parallel mRNA/DNA sequencing shows major differential alterations in severe COVID-19

Guillaume Monneret
Fabienne Venet

Résumé

To understand the fine differential elements that can lead to or prevent acute respiratory distress syndrome (ARDS) in COVID-19 patients, it is crucial to investigate the immune response architecture. We herein dissected the multiple layers of B cell responses by flow cytometry and Ig repertoire analysis from acute phase to recovery. Flow cytometry with FlowSOM analysis showed major changes associated with COVID-19 inflammation such as an increase of double-negative B-cells and ongoing plasma cell differentiation. This paralleled COVID-19-driven expansion of two disconnected B-cell repertoires. Demultiplexing successive DNA and RNA Ig repertoire patterns characterized an early expansion of IgG1 clonotypes with atypically long and uncharged CDR3, the abundance of this inflammatory repertoire being correlated with ARDS and likely pejorative. A superimposed convergent response included convergent anti-SARS-CoV-2 clonotypes. It featured progressively increasing somatic hypermutation together with normal-length or short CDR3 and it persisted until a quiescent memory B-cell stage after recovery.
Fichier principal
Vignette du fichier
1-s2.0-S2589004223003371-main.pdf (7.1 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

inserm-04041493 , version 1 (22-03-2023)

Identifiants

Citer

Virginie Pascal, Marine Dupont, Paco de Rouault, David Rizzo, Delphine Rossille, et al.. Demultiplexing Ig repertoires by parallel mRNA/DNA sequencing shows major differential alterations in severe COVID-19. iScience, 2023, 26 (3), pp.106260. ⟨10.1016/j.isci.2023.106260⟩. ⟨inserm-04041493⟩
59 Consultations
40 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More