Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2003

Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells.

Résumé

Cell cycle dynamics and localization of condensins--multiprotein complexes involved in late stages of mitotic chromosome condensation--were studied in Xenopus laevis XL2 cell line. Western blot analysis of synchronized cells showed that the ratio of levels of both pEg7 and XCAP-E to beta-tubulin levels remains almost constant from G1 to M phase. pEg7 and XCAP-E were localized to the mitotic chromosomes and were detected in interphase nuclei. Immunostaining for condensins and nucleolar proteins UBF, fibrillarin and B23 revealed that both XCAP-E and pEg7 are localized in the granular component of the nucleolus. Nucleolar labeling of both proteins is preserved in segregated nucleoli after 6 hours of incubation with actinomycin D (5 mg/ml), but the size of the labeled zone was significantly smaller. The data suggest a novel interphase function of condensin subunits in spatial organization of the nucleolus and/or ribosome biogenesis.
Fichier non déposé

Dates et versions

inserm-00467029 , version 1 (25-03-2010)

Identifiants

Citer

Rustem Uzbekov, Elmira Timirbulatova, Erwan Watrin, Fabien Cubizolles, David Ogereau, et al.. Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells.. Journal of Cell Science, 2003, 116 (Pt 9), pp.1667-78. ⟨10.1242/jcs.00311⟩. ⟨inserm-00467029⟩
131 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More