Prokineticin 2/Bv8 is expressed in Kupffer cells in liver and is down regulated in human hepatocellular carcinoma. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue World Journal of Gastroenterology Année : 2008

Prokineticin 2/Bv8 is expressed in Kupffer cells in liver and is down regulated in human hepatocellular carcinoma.

Résumé

AIM: To study the implication of prokineticin 1 (PK1/EG-VEGF) and prokineticin 2 (PK2/Bv8) in hepatocellular carcinoma angiogenesis. METHODS: The gene induction of PK1/EG-VEGF and PK2/Bv8 was investigated in 10 normal, 28 fibrotic and 28 tumoral livers by using real time PCR. Their expression was compared to the expression of VEGF (an angiogenesis marker), vWF (an endothelial cell marker) and to CD68 (a monocyte/macrophage marker). Furthermore, the mRNA levels of PK1/EG-VEGF, PK2/Bv8, prokineticin receptor 1 and 2 were evaluated by real time PCR in isolated liver cell populations. Finally, PK2/Bv8 protein was detected in normal liver paraffin sections and in isolated liver cells by immunohistochemistry and immunocytochemistry. RESULTS: PK2/Bv8 mRNA but not PK1/EG-VEGF was expressed in all types of normal liver samples examined. In the context of liver tumor development, we reported that PK2/Bv8 correlates only with CD68 and showed a significant decrease in expression as the pathology evolves towards cancer. Whereas, VEGF and vWF mRNA were significantly upregulated in both fibrosis and HCC, as expected. In addition, out of all isolated liver cells examined, only Kupffer cells (liver resident macrophages) express significant levels of PK2/Bv8 and its receptors, prokineticin receptor 1 and 2. CONCLUSION: In normal liver PK2/Bv8 and its receptors were specifically expressed by Kupffer cells. PK2/Bv8 expression decreased as the liver evolves towards cancer and did not correlate with HCC angiogenesis.

Domaines

Immunologie
Fichier principal
Vignette du fichier
WJG-PK1-PK2_HCC_Figures_table_Monnier_et.al.pdf (3.18 Mo) Télécharger le fichier
Monnier_et_al_WJG_2008_9920.pdf (229.09 Ko) Télécharger le fichier

Dates et versions

inserm-00261475 , version 1 (10-11-2009)

Identifiants

  • HAL Id : inserm-00261475 , version 1
  • PUBMED : 18300343

Citer

Justin Monnier, Claire Piquet-Pellorce, Jean-Jacques Feige, Orlando Musso, Bruno Clément, et al.. Prokineticin 2/Bv8 is expressed in Kupffer cells in liver and is down regulated in human hepatocellular carcinoma.. World Journal of Gastroenterology, 2008, 14 (8), pp.1182-91. ⟨inserm-00261475⟩
202 Consultations
385 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More