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                <title level="j">International Journal of Mass Spectrometry</title>
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                  <biblScope unit="volume">515</biblScope>
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                  <date type="datePub">2025-09</date>
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                <term xml:lang="en">Lichen</term>
                <term xml:lang="en">Polysaccharide</term>
                <term xml:lang="en">Hydrolysis</term>
                <term xml:lang="en">MALDI-TOF MS</term>
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              <p>MALDI-TOF MS methods coupled with offline chromatographic data were used to compare the distribution of oligosaccharides generated from acidic or oxidative degradation (Fitdog) of high molecular weight polysaccharides (&amp;gt;10 kDa) obtained from two lichens Lasallia pustulata and Cetraria islandica. MALDI allowed to quickly compare the kinetics of degradation on both models (starting from non-purified polysaccharides) and to evaluate the dispersity of the resulting oligosaccharides. MALDI-MS confirmed on one hand that TFA hydrolysis gave neutral oligosaccharides easy to correlate with the chemical formula. On the other hand, more structural diversity was evidenced using the Fitdog protocol. Deep analysis of the MALDI data highlighted the formation of by-products corresponding to modified oligosaccharides (e.g., intracyclic cleavages).</p>
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