Comparison of four DNA extraction kits efficiency for 16SrDNA microbiota profiling of diverse human samples - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Future Science OA Année : 2023

Comparison of four DNA extraction kits efficiency for 16SrDNA microbiota profiling of diverse human samples

Résumé

Aim: The current study investigated the performance of 4 widely used DNA extraction kits using different types of high (stool) and low biomass samples (chyme, broncho alveolar lavage and sputum). Methods: Qiagen Powerfecal Pro DNA kit, Macherey Nucleospin Soil kit, Macherey Nucleospin Tissue Kit and MagnaPure LC DNA isolation kit III were evaluated in terms of DNA quantity, quality, diversity and composition profiles. Results: The quantity and quality of DNA varied among the four kits. The microbiota of the stool samples showed similar diversity and compositional profiles for the 4 kits. Conclusion: Despite differences in DNA quality and quantity, the 4 kits yielded similar results for stool samples, while all kits were not sensitive enough for low biomass samples.
Fichier principal
Vignette du fichier
hal-04049534.pdf (944.61 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04049534 , version 1 (30-05-2023)

Licence

Paternité

Identifiants

Citer

Sandrine Le Gall-David, Gaëlle Boudry, Sylvie Buffet-Bataillon. Comparison of four DNA extraction kits efficiency for 16SrDNA microbiota profiling of diverse human samples. Future Science OA, 2023, 9 (1), pp.FSO837. ⟨10.2144/fsoa-2022-0072⟩. ⟨hal-04049534⟩
20 Consultations
17 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More