%0 Journal Article %T Combined effects of high fat diet and exercise on autophagy in white adipose tissue of mice %+ Islamic Azad University of Hamedan %+ Tehran University of Medical Sciences (TUMS) %+ University of Guilan %+ Alzahra University %+ University of British Columbia (UBC) %+ University of Kurdistan [Sanandaj - Iran] (UOK) %+ University of Freiburg [Freiburg] %+ Laboratoire Mouvement Sport Santé (M2S) %+ Institut International des Sciences du Sport (2I2S) %A Daneshyar, Saeed %A Tavoosidana, Gholamreza %A Bahmani, Mahdi %A Basir, Saeed Shokati %A Delfan, Maryam %A Laher, Ismail %A Saeidi, Ayoub %A Granacher, Urs %A Zouhal, Hassane %Z This work was supported by Tehran University of Medical Sciences and Health Services (no. 47823) and University of Ayatollah Alozma Boroujerdi (no. 15664-214256). %< avec comité de lecture %@ 0024-3205 %J Life Sciences %I Elsevier %V 314 %P 121335 %8 2023-02 %D 2023 %R 10.1016/j.lfs.2022.121335 %M 36587790 %K Adipose tissue %K Autophagy %K Exercise training %K High-fat diet %K Obesity %Z Life Sciences [q-bio]Journal articles %X Aim: The effects of nutrition and exercise on autophagy are not well studied. This study aimed to investigate the combined effects of high-fat diets (HFD) and exercise training (ET) on autophagy in white adipose tissue of mice.Materials and methods: Male C57BL/6 mice were assigned into four groups of 7 mice per group: (1) Control, (2) high-fat diet-induced obesity (HFD-Ob), (3) exercise training (ET), and (4) high-fat diet with exercise training (HFD-ET). The HFD-Ob group was fed a high-fat diet for 14 weeks, while the ET group continuously ran on a treadmill for five sessions per week for seven weeks, and the HFD-ET group had both HFD and exercise training. qReal-time-PCR and western blot were used to measure the mRNA and protein levels of autophagy markers in white adipose tissue.Results: Mice from the HFD group showed higher levels in autophagy-related gene5 (ATG5, p = 0.04), ATG7 (p = 0.002), cathepsin B (CTSB, p = 0.0004), LC3-II (p = 0.03) than control. Mice in the ET group displayed higher levels of genes for ATG7 (p = 0.0003), microtubule-associated protein1-light chain 3 (LC3, p = 0.05), lysosome-associated membrane protein 2 (LAMP2, p = 0.04) and cathepsin L (CTSL, p = 0.03) than control. Mice from the HFD-ET group had higher levels of genes for ATG7 (p = 0.05) and CTSL (p = 0.043) and lower levels of genes for CTSB (p = 0.045) compared to the HFD group and lower levels of LAMP2 (p = 0.02) compared to the ET group.Conclusion: There were increases in autophagosome formation in the white adipose tissue from mice in the HFD and ET groups. A combination of HFD and ET enhances autophagosome formation and modulates lysosomal degradation in white adipose tissue. %G English %2 https://univ-rennes.hal.science/hal-03930680/document %2 https://univ-rennes.hal.science/hal-03930680/file/Daneshyar%20et%20al.%20-%202022%20-%20Combined%20effects%20of%20high%20fat%20diet%20and%20exercise%20on%20.pdf %L hal-03930680 %U https://univ-rennes.hal.science/hal-03930680 %~ UNIV-BREST %~ UNIV-RENNES1 %~ UR2-HB %~ IFR140 %~ STATS-UR1 %~ M2S %~ UR1-HAL %~ UR1-SHS %~ UNIV-RENNES2 %~ TEST-UR-CSS %~ UNIV-RENNES %~ UR1-BIO-SA