Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2021

Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging

Xavier Morin

Résumé

Biocompatible fluorescent reporters with spectral properties spanning the entire visible spectrum are indispensable tools for imaging the biochemistry of living cells and organisms in real time. Here, we report the engineering of a fluorescent chemogenetic reporter with tunable optical and spectral properties. A collection of fluorogenic chromophores with various electronic properties enables to generate bimolecular fluorescent assemblies that cover the visible spectrum from blue to red using a single protein tag engineered and optimized by directed evolution and rational design. The ability to tune the fluorescence color and properties through simple molecular modulation provides a broad experimental versatility for imaging proteins in live cells, including neurons, and in multicellular organisms, and opens avenues for optimizing Förster resonance energy transfer (FRET) biosensors in live cells. The ability to tune the spectral properties and fluorescence performance enables furthermore to match the specifications and requirements of advanced super-resolution imaging techniques.
Fichier principal
Vignette du fichier
s41467-021-27334-0.pdf (12.3 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

hal-03463472 , version 1 (02-12-2021)

Licence

Paternité

Identifiants

Citer

Hela Benaissa, Karim Ounoughi, Isabelle Aujard, Evelyne Fischer, Rosette Goïame, et al.. Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging. Nature Communications, 2021, 12 (1), pp.6989. ⟨10.1038/s41467-021-27334-0⟩. ⟨hal-03463472⟩
85 Consultations
69 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More