Extracellular vesicles shed by follicular lymphoma B cells promote the polarization of bone marrow stromal cell niche - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Blood Année : 2021

Extracellular vesicles shed by follicular lymphoma B cells promote the polarization of bone marrow stromal cell niche

Damarys Loew
Florent Dingli

Résumé

Follicular Lymphoma (FL) originates in the lymph nodes (LN) and infiltrates bone marrow (BM) early in the course of the disease. BM FL B cells are characterized by a lower cytological grade, a decreased proliferation, and a specific phenotypic and subclonal profile. Mesenchymal stromal cells (MSC) obtained from FL BM display a specific gene expression profile (GEP), including enrichment for a lymphoid-stromal cell signature, and an increased capacity to sustain FL B-cell growth. However, the mechanisms triggering the formation of the medullar FL permissive stromal niche have not been yet identified. In the current work, we demonstrated that FL B cells produced extracellular vesicles (EVs) that could be internalized by BM-MSC, making them more efficient to support FL B-cell survival and quiescence. Accordingly, EVs purified from FL BM plasma activated TGF-b dependent and independent pathways in BM-MSC, modified their GEP, triggering an upregulation of factors classically associated with hematopoietic stem cell niche, including CXCL12 or angiopoietin-1. Moreover, we provided the first characterization of BM FL B-cell GEP, allowing the definition of the landscape of molecular interactions they could engage with EV-primed BM-MSC. This work identified FL-derived EVs as putative mediators of BM stroma polarization and supported further investigation of their clinical interest for targeting the crosstalk between BM-MSC and malignant B cells.

Domaines

Cancer Hématologie
Fichier principal
Vignette du fichier
Dumontet et al-2021-Extracellular vesicles shed by follicular lymphoma B cells promote the.pdf (9.93 Mo) Télécharger le fichier
Dumontet - Supplemental_Reviewing_final_preprint.pdf (7.8 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03225033 , version 1 (19-05-2021)

Identifiants

Citer

Erwan Dumontet, Céline Pangault, David Roulois, Matthis Desoteux, Simon Léonard, et al.. Extracellular vesicles shed by follicular lymphoma B cells promote the polarization of bone marrow stromal cell niche. Blood, 2021, 138 (1), pp.57-70. ⟨10.1182/blood.2020008791⟩. ⟨hal-03225033⟩
319 Consultations
283 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More