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Article Dans Une Revue Zebrafish Année : 2020

A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos

Résumé

In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consumption in living zebrafish larvae using a spectrofluorometer and the MitoXpress Xtra Oxygen Consumption Assay. In addition, we used zebrafish larvae treated with mitochondrial respiratory chain inhibitors, antimycin A or rotenone, to verify that our method enables precise and reliable measurements of oxygen consumption.
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hal-03097083 , version 1 (05-01-2021)

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Julie Somkhit, Roxane Loyant, Alexandre Brenet, Rahma Hassan-Abdi, Constantin Yanicostas, et al.. A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos. Zebrafish, 2020, 17 (4), pp.268-270. ⟨10.1089/zeb.2020.1878⟩. ⟨hal-03097083⟩
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