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Article Dans Une Revue Molecular Ecology Resources Année : 2009

Development of nine polymorphic microsatellite markers for the phytoparasitic nematode Xiphinema index, the vector of the grapevine fanleaf virus

Résumé

We report isolation, characterization and cross-species amplification of nine microsatellite loci from the phytoparasitic nematode Xiphinema index, the vector of grapevine fanleaf virus. Levels of polymorphism were evaluated in 62 individuals from two X. index populations. The number of alleles varies between two and 10 depending on locus and population. Observed heterozygosity on loci across both populations varied from 0.32 to 0.857 (mean 0.545). The primers were tested for cross-species amplification in three other species of phytoparasitic nematodes of the Xiphinema genus. These nine microsatellite loci constitute valuable markers for population genetics and phylogeographical studies of X. index

Dates et versions

hal-02658582 , version 1 (30-05-2020)

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Laure Villate, Daniel Esmenjaud, Solene Coedel, Olivier Plantard. Development of nine polymorphic microsatellite markers for the phytoparasitic nematode Xiphinema index, the vector of the grapevine fanleaf virus. Molecular Ecology Resources, 2009, 9 (1), pp.229-232. ⟨10.1111/j.1755-0998.2008.02411.x⟩. ⟨hal-02658582⟩
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