%0 Journal Article %T Sub-chronic exposure to Kalach 360 SL, Glyphosate-based Herbicide, induced bone rarefaction in female Wistar rats %+ Université de Sfax - University of Sfax %+ Institut des Sciences Chimiques de Rennes (ISCR) %+ Hopital Habib Bourguiba - Habib Bourguiba Hospital [Sfax] %A Hamdaoui, Latifa %A Oudadesse, Hassane %A Lefeuvre, Bertrand %A Mahmoud, Asma %A Naifer, Manel %A Badraoui, Riadh %A Ayadi, Fatma %A Rebai, Tarek %Z This work was supported by grants from the Tunisian Ministry of Higher Education and Scientific Researchand it was carried out in the research unit 12ES15 (previously 99/UR/0860) of the Faculty of Medicine, Sfax-Tunisia. The authors are grateful to Pr. Wassim Hariz and Pr. Kamel Maaloul English Language Teachers at the Faculty of Science of Sfax, for having proofread the manuscript. %< avec comité de lecture %@ 0300-483X %J Toxicology %I Elsevier %V 436 %P 152412 %8 2020 %D 2020 %R 10.1016/j.tox.2020.152412 %M 32145347 %K Glyphosate %K Kalach 360 SL %K Osteoporosis %K Rats %K Bone tissue %K Bone remodeling %Z Life Sciences [q-bio]/ToxicologyJournal articles %X We investigated the effects of Kalach 360 SL (KL), Glyphosate (G)-based herbicide, on bone tissue in different groups of female Wistar rats. Group 1 (n = 6) received a standard diet and served as a control, groups 2 and 3 (n = 6 each) received 0.07 ml (D1 126 mg/Kg) and 0.175 ml (D2 315 mg/Kg) of KL dissolved in the water for 60 days. The plasma was used to examine the metabolic balance markers (calcium, phosphorus, phosphatase alkaline (PAL), and vitamin D (vit D) and hormonal status (oestrogen and thyroid hormones). As a result, sub-chronic exposure to KL induced a perturbation of bone metabolism (calcium and phosphorus) and hormonal status disturbance. The histological and immunohistochemical study of the thyroid gland revealed a disturbance in morphological structure and thyroid cells function. Moreover, the KL disrupting eff ;ect on thyroid function was investigated by measuring changes in plasma levels of thyroid hormones. Free triiodothyronine (FT3) and thyroxine (FT4) were decreased in female rats breast-fed from rats treated with D and D2 of KL. This eff ;ect was associated with an increase in the plasma level of thyroid-stimulating hormone (TSH). Thus, that KL leads to hypothyroidism. Decrease in levels of oestrogen and thyroid dysfunction led to a disruption in the skeletal bone. The histological study and SEM in bone results allowed us to observe, in rats exposed to KL, the thinning and discontinuity of bone trabecular with a significant decrease in the number of nodes (intertrabecular links).In conclusion, KL sub-chronic exposure caused an aspect of osteoporosis. %G English %2 https://univ-rennes.hal.science/hal-02533168/document %2 https://univ-rennes.hal.science/hal-02533168/file/Hamdaoui%20et%20al-2020-Sub-chronic%20exposure%20to%20Kalach%20360%20SL%2C%20Glyphosate%20-based%20Herbicide%2C%20induced.pdf %L hal-02533168 %U https://univ-rennes.hal.science/hal-02533168 %~ UNIV-RENNES1 %~ CNRS %~ INSA-RENNES %~ ENSC-RENNES %~ ISCR %~ SCR_VC %~ SCR_OMC2012 %~ ISCR-OMC %~ STATS-UR1 %~ UR1-SPM %~ INC-CNRS %~ UR1-UFR-SPM %~ UR1-HAL %~ UR1-SDLM %~ IVER %~ TEST-UR-CSS %~ UNIV-RENNES %~ INSA-GROUPE %~ TEST-HALCNRS %~ UR1-MMS %~ TEST2-HALCNRS