Myelinosome-like vesicles in human seminal plasma A cryo-electron microscopy study - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Cryobiology Année : 2020

Myelinosome-like vesicles in human seminal plasma A cryo-electron microscopy study

Résumé

Seminal plasma is particularly rich in extracellular vesicles. Myelinosomes are membranous organelles described throughout the seminiferous epithelium of the testis but never reported in semen. Our aim was to determine the presence of myelinosomes in human seminal plasma. Transmission electron microscopy and cryo electron microscopy analysis of standard myelinosome preparation from TM4 Sertoli cells and human seminal plasma samples. We have specified by cryo-EM the morphological aspect of "standard" myelinosomes isolated from the culture media of TM4 Sertoli cells. Vesicles with the same morphological appearance were revealed in human seminal plasma samples. Human seminal plasma contains a population of large EV (average diameter 200 nm) whose morphological appearance resemble those of myelinosomes. Defining the specific biomarkers and functionalities of myelinosome in human seminal plasma are the concerns to be addressed in our further research.
Fichier principal
Vignette du fichier
Yefimova et al-2019-.pdf (12.42 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02304994 , version 1 (27-05-2020)

Identifiants

Citer

M Yefimova, E Bere, A. S. Neyroud, Bernard Jégou, Nicolas Bourmeyster, et al.. Myelinosome-like vesicles in human seminal plasma A cryo-electron microscopy study. Cryobiology, 2020, 92, pp.15-20. ⟨10.1016/j.cryobiol.2019.09.009⟩. ⟨hal-02304994⟩
172 Consultations
152 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More