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Article Dans Une Revue Biophysical Journal Année : 2001

Coherent Scattering in Multi-Harmonic Light Microscopy

Résumé

By focusing a pulsed laser beam into a sample, harmonic up-conversion can be generated as well as multi-photon excited fluorescence. Whereas multi-photon excited fluorescence microscopy is well established, the use of multi-harmonic generation for three-dimensional image contrast is very recent. Both techniques can provide similar resolution and, for adequate radiating source density, comparable signal levels, allowing them to be combined in a single versatile instrument. However, harmonic generation differs fundamentally from fluorescence generation in that it is coherent and produces radiation patterns that are highly sensitive to phase. As such, multi-harmonic generation microscopy provides a unique window into molecular spatial organization that is inaccessible to fluorescence.
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Dates et versions

hal-01920233 , version 1 (13-11-2018)

Identifiants

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Laurent Moreaux, Olivier Sandre, Serge Charpak, Mireilles Blanchard-Desce, Jérôme Mertz. Coherent Scattering in Multi-Harmonic Light Microscopy. Biophysical Journal, 2001, 80 (3), pp.1568-1574. ⟨10.1016/S0006-3495(01)76129-2⟩. ⟨hal-01920233⟩
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