%0 Journal Article %T New transcriptomic tools to understand testis development and functions %+ Institut de recherche en santé, environnement et travail (Irset) %A Lecluze, Estelle %A Jégou, Bernard %A Rolland, Antoine D. %A Chalmel, Frédéric %Z Institut National de la Santé et de la Recherche Médicale %Z Université de Rennes 1 %Z CRS115_171007, Swiss National Foundation %Z Swiss National Foundation (SNF) %Z Research Institute for Environmental and Occupational Health (Irset) %Z French School of Public Health (EHESP) %< avec comité de lecture %@ 0303-7207 %J Molecular and Cellular Endocrinology %I Elsevier %V 468 %P 47-59 %8 2018-06 %D 2018 %R 10.1016/j.mce.2018.02.019 %M 29501799 %K Spermatogenesis %K Germ cells %K RNA-sequencing %K Testis %K Development %K Transcriptomics %Z Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] %Z Life Sciences [q-bio]/Development Biology/GametogenesisJournal articles %X The testis plays a central role in the male reproductive system - secreting several hormones including male steroids and producing male gametes. A complex and coordinated molecular program is required for the proper differentiation of testicular cell types and maintenance of their functions in adulthood. The testicular transcriptome displays the highest levels of complexity and specificity across all tissues in a wide range of species. Many studies have used high-throughput sequencing technologies to define the molecular dynamics and regulatory networks in the testis as well as to identify novel genes or gene isoforms expressed in this organ. This review intends to highlight the complementarity of these transcriptomic studies and to show how the use of different sequencing protocols contribute to improve our global understanding of testicular biology. %G English %2 https://univ-rennes.hal.science/hal-01864339/document %2 https://univ-rennes.hal.science/hal-01864339/file/Lecluze%20et%20al_New%20transcriptomic%20tools_R1_v8.pdf %L hal-01864339 %U https://univ-rennes.hal.science/hal-01864339 %~ INSERM %~ UNIV-RENNES1 %~ UNIV-ANGERS %~ IFR140 %~ IRSET %~ STATS-UR1 %~ IRSET-SMS %~ IRSET-CCII %~ IRSET-TREC %~ IRSET-HIAEC %~ IRSET-SMLF %~ IRSET-ERD %~ IRSET-VCER %~ IRSET-NEED %~ IRSET-TNGC %~ UR1-UFR-SVE %~ UR1-HAL %~ EHESP %~ USPC %~ UR1-SDV %~ IRSET-8 %~ IRSET-EHESP %~ DSETGS %~ TEST-UR-CSS %~ UNIV-RENNES %~ UR1-BIO-SA