%0 Journal Article %T Concentrating mixtures of neuroactive pharmaceuticals and altered neurotransmitter levels in the brain of fish exposed to a wastewater effluent %+ University of Sussex %+ University of Exeter %A David, Arthur %A Lange, Anke %A Tyler, Charles R. %A Hill, Elizabeth M. %Z Marie Curie Intra European Fellowship within European Community Seventh Framework Programme [302097] %< avec comité de lecture %@ 0048-9697 %J Science of the Total Environment %I Elsevier %V 621 %P 782-790 %8 2018-04 %D 2018 %R 10.1016/j.scitotenv.2017.11.265 %M 29202289 %K Fish %K Antidepressants %K Antipsychotics %K SSRIs %K Neurotransmitters %K Wastewater effluents %Z Life Sciences [q-bio]/Santé publique et épidémiologieJournal articles %X Fish can be exposed to a variety of neuroactive pharmaceuticals via the effluent discharges from wastewater treatment plants and concerns have arisen regarding their potential impacts on fish behaviour and ecology. In this study, we investigated the uptake of 14 neuroactive pharmaceuticals from a treated wastewater effluent into blood plasma and brain regions of roach (Rutilus rutilus) after exposure for 15 days. We show that a complex mixture of pharmaceuticals including, 6 selective serotonin reuptake inhibitors, a serotonin-noradrenaline reuptake inhibitor, 3 atypical antipsychotics, 2 tricyclic antidepressants and a benzodiazepine, concentrate in different regions of the brain including the telencephalon, hypothalamus, optic tectum and hindbrain of effluent-exposed fish. Pharmaceuticals, with the exception of nordiazepam, were between 3-40 fold higher in brain compared with blood plasma, showing these neuroactive drugs are readily uptaken, into brain tissues in fish. To assess for the potential for any adverse ecotoxicological effects, the effect ratio was calculated from human therapeutic plasma concentrations (HtPCs) and the measured or predicted fish plasma concentrations of pharmaceuticals. After accounting for a safety factor of 1000, the effect ratios indicated that fluoxetine, norfluoxetine, sertraline, and amitriptyline warrant prioritisation for risk assessment studies. Furthermore, although plasma concentrations of all the pharmaceuticals were between 33 and 5714-fold below HtPCs, alterations in serotonin, glutamate, acetylcholine and tryptophan concentrations were observed in different brain regions of effluent-exposed fish. This study highlights the importance of determining the potential health effects arising from the concentration of complex environmental mixtures in risk assessment studies. (c) 2017 Elsevier B.V. All rights reserved. %G English %L hal-01737329 %U https://univ-rennes.hal.science/hal-01737329 %~ SANTE_PUB_INSERM %~ IRSET %~ STATS-UR1 %~ UR1-UFR-SVE %~ UR1-SDV %~ UNIV-RENNES %~ UR1-BIO-SA