%0 Journal Article %T Aurora A activation in mitosis promoted by BuGZ %+ Department of Embryology [Carnegie] %+ Indiana University [Bloomington] %+ Institut de Génétique et Développement de Rennes (IGDR) %+ Shanghai Institutes for Biological Sciences %+ Chinese Academy of Medical Sciences and Peking Union Medical College %A Huang, Yuejia %A Li, Teng %A Ems-Mcclung, Stephanie C. %A Walczak, Claire E. %A Prigent, Claude %A Zhu, Xueliang %A Zhang, Xuemin %A Zheng, Yixian %Z National Institutes of Health [R01 GM056312, R01 GM106023, R01 GM 059618] %Z National Natural Science Foundation of China [31420103916] %Z Ligue Nationale Contre le Cancer %< avec comité de lecture %@ 0021-9525 %J Journal of Cell Biology %I Rockefeller University Press %V 217 %N 1 %P 107-116 %8 2018-01-02 %D 2018 %R 10.1083/jcb.201706103 %M 29074706 %Z Life Sciences [q-bio]/GeneticsJournal articles %X Protein phase separation or coacervation has emerged as a potential mechanism to regulate biological functions. We have shown that coacervation of a mostly unstructured protein, BuGZ, promotes assembly of spindle and its matrix. BuGZ in the spindle matrix binds and concentrates tubulin to promote microtubule (MT) assembly. It remains unclear, however, whether BuGZ could regulate additional proteins to promote spindle assembly. In this study, we report that BuGZ promotes Aurora A (AurA) activation in vitro. Depletion of BuGZ in cells reduces the amount of phosphorylated AurA on spindle MTs. BuGZ also enhances MCAK phosphorylation. The two zinc fingers in BuGZ directly bind to the kinase domain of AurA, which allows AurA to incorporate into the coacervates formed by BuGZ in vitro. Importantly, mutant BuGZ that disrupts the coacervation activity in vitro fails to promote AurA phosphorylation in Xenopus laevis egg extracts. These results suggest that BuGZ coacervation promotes AurA activation in mitosis. %G English %2 https://univ-rennes.hal.science/hal-01688184/document %2 https://univ-rennes.hal.science/hal-01688184/file/107.full.pdf %L hal-01688184 %U https://univ-rennes.hal.science/hal-01688184 %~ UNIV-RENNES1 %~ CNRS %~ IFR140 %~ IGDR %~ STATS-UR1 %~ IGDR-CC %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ TEST-HALCNRS %~ UR1-BIO-SA