%0 Journal Article %T Chemical analysis of the Alphaproteobacterium strain MOLA1416 associated with the marine lichen Lichina pygmaea %+ Institut des Sciences Chimiques de Rennes (ISCR) %+ Observatoire océanologique de Banyuls (OOB) %+ Centre Régional de Mesures Physiques de l'Ouest (CRMPO) %+ Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM) %A Parrot, D. %A Intertaglia, L. %A Jéhan, P. %A Grube, M. %A Suzuki, M.T. %A Tomasi, S. %Z 10-INBS-02-01, CNRS, Centre National de la Recherche Scientifique %Z UPMC, University of Pittsburgh Medical Center %< avec comité de lecture %@ 0031-9422 %J Phytochemistry %I Elsevier %V 145 %P 57-67 %8 2018 %D 2018 %R 10.1016/j.phytochem.2017.10.005 %M 29091816 %K Alphaproteobacteria %K Dereplication workflow %K LC/MSn %K Lichen-associated bacteria %K Lichina pygmaea %K Molecular networking %Z Chemical SciencesJournal articles %X Alphaproteobacterium strain MOLA1416, related to Mycoplana ramosa DSM 7292 and Chelativorans intermedius CC-MHSW-5 (93.6% 16S rRNA sequence identity) was isolated from the marine lichen, Lichina pygmaea and its chemical composition was characterized by a metabolomic network analysis using LC-MS/MS data. Twenty-five putative different compounds were revealed using a dereplication workflow based on MS/MS signatures available through GNPS (https://gnps.ucsd.edu/). In total, ten chemical families were highlighted including isocoumarins, macrolactones, erythrinan alkaloids, prodiginines, isoflavones, cyclohexane-diones, sterols, diketopiperazines, amino-acids and most likely glucocorticoids. Among those compounds, two known metabolites (13 and 26) were isolated and structurally identified and metabolite 26 showed a high cytotoxic activity against B16 melanoma cell lines with an IC50 0.6 ± 0.07 μg/mL. © 2017 Elsevier Ltd %G English %2 https://univ-rennes.hal.science/hal-01639693/document %2 https://univ-rennes.hal.science/hal-01639693/file/PHYTO_11662_edit_report.pdf %L hal-01639693 %U https://univ-rennes.hal.science/hal-01639693 %~ UNIV-RENNES1 %~ CNRS %~ INSA-RENNES %~ ENSC-RENNES %~ ISCR %~ SCR-PNSCM %~ LBBM %~ STATS-UR1 %~ UR1-SPM %~ INC-CNRS %~ UR1-UFR-SPM %~ UR1-HAL %~ ISCR-CORINT %~ UR1-SDLM %~ EDF %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ INSA-GROUPE %~ SORBONNE-UNIVERSITE %~ SORBONNE-UNIV %~ SU-SCIENCES %~ SU-SCI %~ UMS-2348 %~ ISCR-CORINT2 %~ TEST-HALCNRS %~ SU-TI %~ UR1-MMS %~ ISCR-CORINT3 %~ ALLIANCE-SU %~ TEST2-HALCNRS %~ TEST3-HALCNRS