%0 Journal Article %T Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of human oral Capnocytophaga species %+ Nutrition, Métabolismes et Cancer (NuMeCan) %+ CHU Pontchaillou [Rennes] %A Jolivet-Gougeon, Anne %A Helsens, Nicolas %A Renard, Elise %A Tamanai-Shacoori, Zohreh %A Bonnaure-Mallet, Martine %< avec comité de lecture %@ 1075-9964 %J Anaerobe %I Elsevier Masson %V 48 %P 89-93 %8 2017-12 %D 2017 %R 10.1016/j.anaerobe.2017.07.003 %M 28739337 %K cfxA %K Capnocytophaga %K PCR %K MALDI-TOF mass spectrometry %K Dendrogram %K 16S rRNA %Z Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology %Z Life Sciences [q-bio]/BioengineeringJournal articles %X Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for rapid identification of cfxA PCR positive and negative Capnocytophaga strains. Colonies were grown on blood agar, incubated anaerobically at 37 °C for 48 h, and were then evaluated by MALDI-TOF MS and 16S rRNA gene sequencing. Both methods identified all colonies to the genus level. The MALDI-TOF MS method gave the same result, at the species level, as 16S rRNA gene sequencing for 41/53 Capnocytophaga sp. strains (77.4%), but the limit of this technique was the absence of some species (C. leadbetteri, C. AHN) in the Biotyper-Bruker® database used in this study. Distinction between the cefotaxime resistant and susceptible strains was unsuccessful using the MALDI-TOF MS method. This technique had low discriminatory power to rapidly detect beta-lactamase-producing Capnocytophaga strains in clinical samples. However, the results from a score-oriented dendrogram confirmed MALDI-TOF MS is a rapid, inexpensive, and reliable method for Capnocytophaga species identification. Enrichment of the reference database used (Biotyper®) will improve future results. %G English %2 https://univ-rennes.hal.science/hal-01619482/document %2 https://univ-rennes.hal.science/hal-01619482/file/Evaluation%20of%20matrix-assisted%20laser%20desorption_accepted.pdf %L hal-01619482 %U https://univ-rennes.hal.science/hal-01619482 %~ INSERM %~ UNIV-RENNES1 %~ INRA %~ HL %~ STATS-UR1 %~ NUMECAN %~ NUMECAN-CIMIAD %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ AGREENIUM %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ NUMECAN-METHER %~ UR1-BIO-SA