Evaluation of whole-blood conservation reagents for Hematoflow-based WBC differential count: Unsatisfactory results - Université de Rennes Accéder directement au contenu
Article Dans Une Revue International Journal of Laboratory Hematology Année : 2017

Evaluation of whole-blood conservation reagents for Hematoflow-based WBC differential count: Unsatisfactory results

Résumé

Background:As conservation of whole blood samples undergoing white blood cell (WBC) differential performed by flow cytometry (Hematoflow) is needed, we evaluated the effects of two commercially available fixatives, namely TransfixTM and Streck Cell PreservativeTM.Methods:We focused on 15 normal samples and on 13 various pathological samples. We compared the two fixatives and cold- or room- temperature effects on various parameters provided by the Hematoflow system.Results:We observed that, even after 2 hours of sample treatment, the conservative methods led to significant modifications of the cell percentages due to substantial variations of the epitope expression.Conclusion:None of the different conservation methods is really reliable for WBC differential performed by flow cytometry and thus samples should be analyzed promptly or stored at 4°C.

Domaines

Cancer
Fichier principal
Vignette du fichier
Evaluation of whole blood conservation reagents_accepted.pdf (879.4 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01616400 , version 1 (10-11-2017)

Identifiants

Citer

Orianne Wagner-Ballon, Bouchra Badaoui, Audrey Brignoli, Jérôme Le Priol, Mikael Roussel. Evaluation of whole-blood conservation reagents for Hematoflow-based WBC differential count: Unsatisfactory results. International Journal of Laboratory Hematology, 2017, 39 (5), pp.e127-e131. ⟨10.1111/ijlh.12697⟩. ⟨hal-01616400⟩
192 Consultations
417 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More