%0 Journal Article %T The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas %+ Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)) %+ Microenvironment, Cell Differentiation, Immunology and Cancer (MICMAC) %A Le Gallou, Simon %A Nojima, Takuya %A Kitamura, Daisuke %A Weill, Jean-Claude %A Reynaud, Claude-Agnès %< avec comité de lecture %@ 1064-3745 %J Methods in Molecular Biology %I Humana Press/Springer Imprint %V 1623 %P 243-251 %8 2017 %D 2017 %R 10.1007/978-1-4939-7095-7_19 %M 28589361 %K Aicda %K Fate mapping %K Fluorescent reporter %K germinal center %K Memory B cells %Z Life Sciences [q-bio]/Cancer %Z Life Sciences [q-bio]/ImmunologyJournal articles %X Expression of activation-induced cytidine deaminase (AID) is the hallmark of B cells engaged in an immune response in germinal centers. We designed an inducible fate-mapping reporter mouse in which AID-expressing B cells could be timely and irreversibly marked, by knockin at the Aicda locus of a tamoxifen-inducible Cre recombinase. This mouse model allows notably for the long-term follow-up of memory B cells and plasma cells engaged in an immune response. We describe here a protocol to generate hybridomas from small memory subsets that can be easily traced and identified in this mouse line through Cre-activated fluorescent reporters. %G English %L hal-01560459 %U https://univ-rennes.hal.science/hal-01560459 %~ UNIV-PARIS5 %~ UNIV-RENNES1 %~ CNRS %~ IFR140 %~ U917 %~ STATS-UR1 %~ UR1-UFR-SVE %~ UR1-HAL %~ USPC %~ UR1-SDV %~ MICMAC %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ UNIV-PARIS %~ UP-SANTE %~ TEST-HALCNRS %~ UR1-BIO-SA