%0 Journal Article %T Efficient transfection of Xenobiotic Responsive Element-biosensor plasmid using diether lipid and phosphatidylcholine liposomes in differentiated HepaRG cells %+ Institut des Sciences Chimiques de Rennes (ISCR) %+ Nutrition, Métabolismes et Cancer (NuMeCan) %A Demazeau, Maxime %A Quesnot, Nicolas %A Ripoche, Nicolas %A Rauch, Claudine %A Jeftić, Jelena %A Morel, Fabrice %A Gauffre, Fabienne %A Benvegnu, Thierry %A Loyer, Pascal %Z This work was funded by the Agence Nationale de la Recherche (ANR, contract NISTEC ANR-09-CESA-003-002), the Institut National de la santé et de la Recherche Médicale (Inserm) and the Centre National de la Recherche Scientifique (CNRS). %< avec comité de lecture %@ 0378-5173 %J International Journal of Pharmaceutics %I Elsevier %V 524 %N 1-2 %P 268-278 %8 2017-05 %D 2017 %R 10.1016/j.ijpharm.2017.03.080 %M 28365389 %K archaeolipids %K biosensor plasmid %K diether lipids %K HepaRG cells %K Liposomes %K Transfection %Z Chemical Sciences/Medicinal Chemistry %Z Life Sciences [q-bio]/Pharmaceutical sciences/PharmacologyJournal articles %X In this study, we evaluated cationic liposomes prepared from diether-NH2 and egg phosphatidylcholine (EPC) for in vitro gene delivery. The impact of the lipid composition, i.e. the EPC and Diether-NH2 molar ratio, on in vitro transfection efficiency and cytotoxicity was investigated using the human HEK293T and hepatoma HepaRG cells known to be permissive and poorly permissive cells for liposome-mediated gene transfer, respectively. Here, we report that EPC/Diether-NH2-based liposomes enabled a very efficient transfection with low cytotoxicity compared to commercial transfection reagents in both HEK293T and proliferating progenitor HepaRG cells. Taking advantage of these non-toxic EPC/Diether-NH2-based liposomes, we developed a method to efficiently transfect differentiated hepatocyte-like HepaRG cells and a biosensor plasmid containing a Xenobiotic Responsive Element and a minimal promoter driving the transcription of the luciferase reporter gene. We demonstrated that the luciferase activity was induced by a canonical inducer of cytochrome P450 genes, the benzo[a]pyrene, and two environmental contaminants, the fluoranthene, a polycyclic aromatic hydrocarbon, and the endosulfan, an organochlorine insecticide, known to induce toxicity and genotoxicity in differentiated HepaRG cells. In conclusion, we established a new efficient lipofection-mediated gene transfer in hepatocyte-like HepaRG cells opening new perspectives in drug evaluation relying on xenobiotic inducible biosensor plasmids. %G English %2 https://univ-rennes.hal.science/hal-01518045/document %2 https://univ-rennes.hal.science/hal-01518045/file/Demazeau%20et%20al.%20-%20Efficient%20Transfection%20of%20Xenobiotic%20Responsive%20El.pdf %L hal-01518045 %U https://univ-rennes.hal.science/hal-01518045 %~ INSERM %~ UNIV-RENNES1 %~ CNRS %~ INSA-RENNES %~ ENSC-RENNES %~ INRA %~ ISCR %~ SCR_ICMV %~ SCR-COS %~ STATS-UR1 %~ NUMECAN %~ UR1-SPM %~ INC-CNRS %~ NUMECAN-TGTC %~ UR1-UFR-SVE %~ UR1-UFR-SPM %~ UR1-HAL %~ ISCR-CORINT %~ UR1-SDLM %~ UR1-SDV %~ AGREENIUM %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ INSA-GROUPE %~ ISCR-CORINT1 %~ TEST-HALCNRS %~ NUMECAN-METHER %~ ANR %~ UR1-MMS %~ UR1-BIO-SA %~ ISCR-CORINT3 %~ TEST2-HALCNRS