%0 Journal Article %T Definition and identification of small RNA sponges: Focus on miRNA sequestration %+ Institut de Génétique et Développement de Rennes (IGDR) %+ CHU Pontchaillou [Rennes] %+ Institut de Génétique et Développement de Rennes (IGDR) %A Migault, Mélodie %A Donnou-Fournet, Emmanuelle %A Galibert, Marie-Dominique %A Gilot, David %Z We thank Brice Felden for editorial assistance and Laura Bachelot & Arthur Gautron for assistance with the figures and Sébastien Corre for critical reading. This study received financial support from the following: Institut National Du Cancer (Melanoma PAIR program); Ouest Valorisation; Ligue Nationale Contre le Cancer (LNCC et comités Grand-Ouest); Région Bretagne; University of Rennes 1; CNRS; European Organisation for Research and Treatment of Cancer Tournesol program; and the Société Française de Dermatologie. MM received fellowships from ‘Ligue Nationale Contre le Cancer’ (CD-22) and Faculty of Pharmacy of Rennes. EDF received fellowships from ‘Ligue Nationale Contre le Cancer’. The authors thank the Gene Expression and Oncogenesis team for helpful discussions. The authors declare no competing financial interests. %< avec comité de lecture %@ 1046-2023 %J Methods %I Elsevier %V 117 %P 35-47 %8 2017-03 %D 2017 %R 10.1016/j.ymeth.2016.11.012 %M 27876678 %Z Life Sciences [q-bio]/GeneticsJournal articles %X Targeting RNAs appears as an important opportunity to modulate biological processes. Here, we overviewed critical parameters implied in RNAs competition to bind small RNAs. These competitions influence small RNA availability and thereby gene expression and cell fate. We focused on the ability of RNAs to sequester small RNA, mainly the microRNAs (miRNAs) and proposed experimental workflows to demonstrate the existence and activity of RNA-sponge. From this basic science, we detailed tailored oligonucleotides, developed to challenge the binding of small RNA. In vitro and in vivo, these tailored oligonucleotides efficiently restore small RNA activity by preventing their sequestration on RNA-sponges. %G English %2 https://univ-rennes.hal.science/hal-01502131/document %2 https://univ-rennes.hal.science/hal-01502131/file/Migault%20et%20al.%20-%20Definition%20and%20identification%20of%20small%20RNA%20sponges.pdf %L hal-01502131 %U https://univ-rennes.hal.science/hal-01502131 %~ UNIV-RENNES1 %~ CNRS %~ IFR140 %~ HL %~ IGDR %~ STATS-UR1 %~ IGDR-EGO %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ TEST-HALCNRS %~ UR1-BIO-SA