%0 Book Section %T Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L %+ CRLCC Eugène Marquis (CRLCC) %+ Chemistry, Oncogenesis, Stress and Signaling (COSS) %A Poissonnier, Amanda %A Legembre, Patrick %B CD95: Methods and Protocols %S Methods in Molecular Biology %V 1557 %P 117-123 %8 2017 %D 2017 %R 10.1007/978-1-4939-6780-3_12 %M 28078588 %K Boyden chamber %K Cell migration %K Cell motility %K Fas %K FasL %K Metastasis %K PI3K %Z Life Sciences [q-bio]/CancerBook sections %X CD95 receptor, also called Fas or Apo-1, is a member of the tumor necrosis factor receptors (TNF-R) superfamily (Itoh and Nagata, J Biol Chem 268:10932-10937, 1993). Its cognate ligand, CD95L, is a transmembrane cytokine, which can be cleaved by metalloproteases (Matsuno et al., J Rheumatol 28:22-28, 2001; Vargo-Gogola et al., Arch Biochem Biophys 408:155-161, 2002; Kiaei et al., Exp Neurol 205:74-81, 2007; Schulte et al., Cell Death Differ 14:1040-1049, 2007) releasing a soluble ligand into the bloodstream. Recent work has shown that this metalloprotease-cleaved CD95L (cl-CD95L) is involved in carcinogenesis (Malleter et al., Cancer Res 73:6711-6721, 2013). Cl-CD95L also fuels the inflammatory process in patients affected by systemic lupus erythematosus by promoting the accumulation of activated T lymphocytes in enflamed organs (Tauzin et al., PLoS Biol 9:e1001090, 2011). This chapter aims at describing the methodology used to measure the chemoattractive effect of cl-CD95L on human cancer cells and lymphocytes. %G English %L hal-01447231 %U https://univ-rennes.hal.science/hal-01447231 %~ UNIV-RENNES1 %~ FNCLCC %~ MARQUIS %~ STATS-UR1 %~ OSS %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ UR1-BIO-SA