%0 Journal Article %T Metabolic Signature of Remote Ischemic Preconditioning Involving a Cocktail of Amino Acids and Biogenic Amines %+ Biologie Neurovasculaire Intégrée (BNVI) %+ Cardioprotection, Remodelage et Thrombose (CRT) %+ Stress Oxydant et Pathologies Métaboliques (SOPAM) %+ Foie, métabolismes et cancer %+ Service de biochimie et biologie moléculaire %+ Biomarqueurs CArdioNeuroVASCulaires (BioCANVAS) %+ Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI) %A Chao de La Barca, Juan Manuel %A Bakhta, Oussama %A Kalakech, Hussein %A Simard, Gilles %A Tamareille, Sophie %A Catros, Véronique %A Callebert, Jacques %A Gadras, Cedric %A Tessier, Lydie %A Reynier, Pascal %A Prunier, Fabrice %A Mirebeau-Prunier, Delphine %Z Federation Francaise de Cardiologie %Z French Ministry of Education and Research %Z University of Angers %Z University Hospital of Angers %Z Region Pays de la Loire %Z Angers Loire Metropole %< avec comité de lecture %@ 2047-9980 %J Journal of the American Heart Association %I Wiley-Blackwell %V 5 %N 9 %P e003891 %8 2016-09 %D 2016 %R 10.1161/JAHA.116.003891 %M 27664804 %K reperfusion injury %K rat-heart %K l-kynurenine %K cardioprotection %K receptors %K protection %K polyamines %K serotonin %K carnosine %K nitrite %Z Life Sciences [q-bio]/Cancer %Z Life Sciences [q-bio]/Human health and pathology/Hépatology and GastroenterologyJournal articles %X Background-Remote ischemic preconditioning (RIPC) is an attractive therapeutic procedure for protecting the heart against ischemia/reperfusion injury. Despite evidence of humoral mediators transported through the circulation playing a critical role, their actual identities so far remain unknown. We sought to identify plasmatic RIPC-induced metabolites that may play a role. Methods and Results-Rat plasma samples from RIPC and control groups were analyzed using a targeted metabolomic approach aimed at measuring 188 metabolites. Principal component analysis and orthogonal partial least-squares discriminant analysis were used to identify the metabolites that discriminated between groups. Plasma samples from 50 patients subjected to RIPC were secondarily explored to confirm the results obtained in rats. Finally, a combination of the metabolites that were significantly increased in both rat and human plasma was injected prior to myocardial ischemia/reperfusion in rats. In the rat samples, 124 molecules were accurately quantified. Six metabolites (ornithine, glycine, kynurenine, spermine, carnosine, and serotonin) were the most significant variables for marked differentiation between the RIPC and control groups. In human plasma, analysis confirmed ornithine decrease and kynurenine and glycine increase following RIPC. Injection of the glycine and kynurenine alone or in combination replicated the protective effects of RIPC seen in rats. Conclusions-We have hereby reported significant variations in a cocktail of amino acids and biogenic amines after remote ischemic preconditioning in both rat and human plasma. %G English %L hal-01439218 %U https://univ-rennes.hal.science/hal-01439218 %~ UNIV-PARIS5 %~ UNIV-PARIS7 %~ UNIV-PARIS13 %~ UNIV-RENNES1 %~ CNRS %~ UNIV-ANGERS %~ APHP %~ IFR140 %~ U991 %~ U991-E4 %~ STATS-UR1 %~ NUMECAN %~ NUMECAN-TGTC %~ UR1-UFR-SVE %~ UR1-HAL %~ USPC %~ UR1-SDV %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ SORBONNE-PARIS-NORD %~ UNIV-PARIS %~ UR1-BIO-SA %~ TEST3-HALCNRS