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Poster De Conférence Année : 2016

Bitter compounds delayed gastric emptying and induced intestinal smooth muscle relaxation in a pig model

Résumé

Bitter compounds induced innate aversion which was reflected in loss of appetite and decreased fat depositionin a pig model reported by our group. However, the mechanism remains unclear. We hypothesized that bittercompounds would reduce gastric emptying and slow down intestinal peristalsis in the pig. Based on previous invivo trial and known molecular mechanisms, caffeine and quinine were the two bitter compounds selected. Bothcompounds are known agonists of human bitter taste receptors (hT2R) with high homology with the porcine T2Rs: Tas2r4, Tas2r7, Tas2r10, Tas2r20 and Tas2r39. Real-time PCR was used to study the expression ofporcine bitter taste receptor genes in the stomach and duodenum that related to the tested bitter compounds.The results showed high expression both in the stomach and duodenum and that the expression level was mostsignificant in the epithelium relative to the smooth muscle layer. The non-invasive scintigraphy imaging datashowed that caffeine and quinine mixed feed significantly (P<0.01 and P<0.05 respectively) decreased gastricemptying compared to control pigs. In addition, an ex-vivo intestinal smooth muscle contraction study illustratedthat the two bitter compounds induced (P<0.001) dose-response relaxation in the duodenum. To conclude,dietary bitter compounds significantly reduced gastric emptying and gut motility. These effects may partiallyexplain previous results showing a reduction in appetite and fat deposition in a pig model. The potential of bittercompounds to help prevent obesity in humans is worth further investigation.
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Dates et versions

hal-01413522 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-01413522 , version 1
  • PRODINRA : 377811
  • WOS : 000386126000194

Citer

Minghai Fu, Mary-Louise Manchadi, Nadia de Jager, David Val-Laillet, Sylvie Guerin, et al.. Bitter compounds delayed gastric emptying and induced intestinal smooth muscle relaxation in a pig model. 17. International Symposium on Olfaction and Taste (ISOT2016), Jun 2016, Yokohama, Japan. Chemical Senses, 41 (9), 2016, Chemical Senses. ⟨hal-01413522⟩
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