%0 Journal Article %T The cleaved FAS ligand activates the Na+/H+ exchanger NHE1 through Akt/ROCK1 to stimulate cell motility %+ Laboratoire de PhysioMédecine Moléculaire (LP2M) %+ Institut de recherche en santé, environnement et travail (Irset) %+ Oncogenesis Stress Signaling (OSS) %+ Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] (CRCTB) %A Monet, Michaël %A Poet, Mallorie %A Tauzin, Sébastien %A Fouque, Amelie %A Cophignon, Auréa %A Lagadic-Gossmann, Dominique %A Vacher, Pierre %A Legembre, Patrick %A Counillon, Laurent %Z This work was supported by CNRS and the University of Nice-Sophia Antipolis, the ICST Labex, grants from INCa PLBIOL, Ligue Contre le Cancer (Comités d’Ille-et-Vilaine/du Morbihan/des Côtes d’Armor/du Maine et Loire), ARC, Région Bretagne, Rennes Métropole. %< avec comité de lecture %@ 2045-2322 %J Scientific Reports %I Nature Publishing Group %V 6 %N 1 %P 28008 %8 2016-06-15 %D 2016 %R 10.1038/srep28008 %M 27302366 %K Cell migration %K Metastasis %K Cell migration %Z Life Sciences [q-bio]/Cancer %Z Life Sciences [q-bio]/Cellular BiologyJournal articles %X Transmembrane CD95L (Fas ligand) can be cleaved to release a promigratory soluble ligand, cl-CD95L, which can contribute to chronic inflammation and cancer cell dissemination. The motility signaling pathway elicited by cl-CD95L remains poorly defined. Here, we show that in the presence of cl-CD95L, CD95 activates the Akt and RhoA signaling pathways, which together orchestrate an allosteric activation of the Na+/H+ exchanger NHE1. Pharmacologic inhibition of Akt or ROCK1 independently blocks the cl-CD95L-induced migration. Confirming these pharmacologic data, disruption of the Akt and ROCK1 phosphorylation sites on NHE1 decreases cell migration in cells exposed to cl-CD95L. Together, these findings demonstrate that NHE1 is a novel molecular actor in the CD95 signaling pathway that drives the cl-CD95L-induced cell migration through both the Akt and RhoA signaling pathways. %G English %2 https://univ-rennes.hal.science/hal-01334067/document %2 https://univ-rennes.hal.science/hal-01334067/file/Cleaved%20FAS%20ligand%20CC-BY.pdf %L hal-01334067 %U https://univ-rennes.hal.science/hal-01334067 %~ INSERM %~ UNICE %~ UNIV-RENNES1 %~ CNRS %~ UNIV-ANGERS %~ IFR140 %~ FNCLCC %~ MARQUIS %~ IRSET %~ STATS-UR1 %~ IRSET-SMS %~ IRSET-CCII %~ IRSET-TREC %~ IRSET-HIAEC %~ IRSET-SMLF %~ IRSET-ERD %~ IRSET-VCER %~ IRSET-NEED %~ IRSET-TNGC %~ OSS %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ IRSET-3 %~ UNIV-COTEDAZUR %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ IRSET-3-EX-SMS %~ UR1-BIO-SA %~ LP2M