Crossovers get a boost in [i]Brassica[/i] allotriploid and allotetraploid hybrids - Université de Rennes Accéder directement au contenu
Article Dans Une Revue The Plant cell Année : 2010

Crossovers get a boost in [i]Brassica[/i] allotriploid and allotetraploid hybrids

Résumé

Meiotic crossovers are necessary to generate balanced gametes and to increase genetic diversity. Even if crossover number is usually constrained, recent results suggest that manipulating karyotype composition could be a new way to increase rossover frequency in plants. In this study, we explored this hypothesis by analyzing the extent of crossover variation in a set of related diploid AA, allotriploid AAC, and allotetraploid AACC Brassica hybrids. We first used cytogenetic methods to describe the meiotic behavior of the different hybrids. We then combined a cytogenetic estimation of class I crossovers in the entire genome by immunolocalization of a key protein, MutL Homolog1, which forms distinct foci on meiotic chromosomes, with genetic analyses to specifically compare crossover rates between one pair of chromosomes in the different hybrids. Our results showed that the number of crossovers in the allotriploid AAC hybrid was higher than in the diploid AA hybrid. Accordingly, the allotetraploid AACC hybrid showed an intermediate behavior. We demonstrated that this increase was related to hybrid karyotype composition (diploid versus allotriploid versus allotetraploid) and that interference was maintained in the AAC hybrids. These results could provide another efficient way to manipulate recombination in traditional breeding and genetic studies.

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Dates et versions

hal-01203949 , version 1 (31-05-2020)

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Martine Leflon, Laurie Grandont, Frederique Eber, Virginie Huteau, Olivier Coriton, et al.. Crossovers get a boost in [i]Brassica[/i] allotriploid and allotetraploid hybrids. The Plant cell, 2010, 22 (7), pp.2253-2264. ⟨10.1105/tpc.110.075986⟩. ⟨hal-01203949⟩
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