%0 Journal Article %T The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity %+ Institut Jacques Monod (IJM (UMR_7592)) %+ Institut de Génétique et Développement de Rennes (IGDR) %+ Matière et Systèmes Complexes (MSC) %+ Goethe-University Frankfurt am Main %+ Feinberg Cardiovascular Research Institute %A Strale, Pierre-Olivier %A Duchesne, Laurence %A Peyret, Grégoire %A Montel, Lorraine %A Nguyen, Thao %A Png, Evelyn %A Tampé, Robert %A Troyanovsky, Sergey %A Hénon, Sylvie %A Ladoux, Benoit %A Mège, René-Marc %Z This work was supported by grants from Centre National de la Recherche Scientifique, Fondation ARC (R.M. Mège), Human Frontier Science Program grant RPG0040/2012 (B. Ladoux and R.M. Mège), Agence Nationale de la Recherche (ANR 2010 Blan1515 [B. Ladoux and R.M. Mège] and Nanotechnology Program [B. Ladoux]), and European Research Council (ERC) under the European Union’s Seventh Framework Program (FP7/2007-2013)/ERC grant agreement no.617233 (B. Ladoux). Financial supports were from Mechanobiology Institute and Institut Universitaire de France to B. Ladoux. S. Troyanovsky’s work was supported by grant AR44016 from the National Institutes of Health. G. Peyret, P.-O. Strale, and L. Duchesne were supported by fellowships from the University Paris-Diderot, Agence Nationale de la Recherche, and Fondation ARC, respectively. %< avec comité de lecture %@ 0021-9525 %J Journal of Cell Biology %I Rockefeller University Press %V 210 %N 2 %P 333-346 %8 2015 %D 2015 %R 10.1083/jcb.201410111 %M 26195669 %Z Life Sciences [q-bio] %Z Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]Journal articles %X Oligomerization of cadherins could provide the stability to ensure tissue cohesion. Cadherins mediate cell-cell adhesion by forming trans-interactions. They form cis-interactions whose role could be essential to stabilize intercellular junctions by shifting cadherin clusters from a fluid to an ordered phase. However, no evidence has been provided so far for cadherin oligomerization in cellulo and for its impact on cell-cell contact stability. Visualizing single cadherins within cell membrane at a nanometric resolution, we show that E-cadherins arrange in ordered clusters, providing the first demonstration of the existence of oligomeric cadherins at cell-cell contacts. Studying the consequences of the disruption of the cis-interface, we show that it is not essential for adherens junction formation. Its disruption, however, increased the mobility of junctional E-cadherin. This destabilization strongly affected E-cadherin anchoring to actin and cell-cell rearrangement during collective cell migration, indicating that the formation of oligomeric clusters controls the anchoring of cadherin to actin and cell-cell contact fluidity. %G English %L hal-01196599 %U https://univ-rennes.hal.science/hal-01196599 %~ UNIV-PARIS7 %~ UNIV-RENNES1 %~ CNRS %~ IJM %~ IFR140 %~ IGDR %~ STATS-UR1 %~ IGDR-TIPS %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ UNIV-PARIS %~ UP-SCIENCES %~ ANR %~ UR1-BIO-SA %~ MSC-LAB