%0 Journal Article %T Neutrophils trigger a NF-κB dependent polarization of tumor-supportive stromal cells in germinal center B-cell lymphomas %+ Microenvironnement et cancer (MiCa) %+ Etablissement Français du Sang %+ Etablissement Français du Sang Bretagne %+ Etablissement français du sang [Rennes] (EFS Bretagne) %+ Laboratoire Traitement du Signal et de l'Image (LTSI) %+ Service de chirurgie thoracique cardiaque et vasculaire [Rennes] = Thoracic and Cardiovascular Surgery [Rennes] %A Grégoire, Murielle %A Guilloton, Fabien %A Pangault, Céline %A Mourcin, Frédéric %A Sok, Phaktra %A Latour, Maelle %A Amé-Thomas, Patricia %A Flécher, Erwan %A Fest, Thierry %A Tarte, Karin %< avec comité de lecture %@ 1949-2553 %J Oncotarget %I Impact journals %V 6 %N 18 %P 16471--16487 %8 2015-05-12 %D 2015 %R 10.18632/oncotarget.4106 %M 26158216 %K cell interaction %K cell differentiation %K B-cell lymphoma %K lymph node %K tumor microenvironment %Z Life Sciences [q-bio]Journal articles %X Both tumor-associated neutrophils (TAN) and cancer-associated fibroblasts (CAFs) display specific phenotypic and functional features and contribute to tumor cell niche. However, their bidirectional crosstalk has been poorly studied, in particular in the context of hematological malignancies. Follicular lymphomas (FL) and diffuse large B-cell lymphomas (DLBCL) are two germinal center-derived lymphomas where various cell components of infiltrating microenvironment, including TAN and CAFs, have been demonstrated to favor directly and indirectly malignant B-cell survival, growth, and drug resistance. We show here that, besides a direct and contact-dependent supportive effect of neutrophils on DLBCL B-cell survival, mediated through the BAFF/APRIL pathway, neutrophils and stromal cells cooperate to sustain FL B-cell growth. This cooperation relies on an overexpression of IL-8 by lymphoma-infiltrating stromal cells that could thereafter efficiently promote neutrophil survival and prime them to neutrophil extracellular trap. Conversely, neutrophils are able to activate stromal cells in a NF-κB-dependent manner, inducing their commitment towards an inflammatory lymphoid stroma phenotype associated with an increased capacity to trigger malignant B-cell survival, and to recruit additional monocytes and neutrophils through the release of CCL2 and IL-8, respectively. Altogether, a better understanding of the lymphoma-supporting effects of neutrophils could be helpful to design new anti-tumor therapeutic strategies %G English %L hal-01187405 %U https://univ-rennes.hal.science/hal-01187405 %~ UNIV-RENNES1 %~ LTSI %~ IFR140 %~ HL %~ U917 %~ STATS-UR1 %~ LNPRM %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-MATH-STIC %~ UR1-SDV %~ MICMAC %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ UR1-MATH-NUM %~ UR1-BIO-SA