Genome-wide screen identifies a novel p97/CDC-48-dependent pathway regulating ER-stress-induced gene transcription - Université de Rennes Accéder directement au contenu
Article Dans Une Revue EMBO Reports Année : 2015

Genome-wide screen identifies a novel p97/CDC-48-dependent pathway regulating ER-stress-induced gene transcription

Résumé

The accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the Unfolded Protein Response (UPR(ER)) to restore ER homeostasis. The AAA(+) ATPase p97/CDC-48 plays key roles in ER stress by promoting both ER protein degradation and transcription of UPR(ER) genes. Although the mechanisms associated with protein degradation are now well established, the molecular events involved in the regulation of gene transcription by p97/CDC-48 remain unclear. Using a reporter-based genome-wide RNAi screen in combination with quantitative proteomic analysis in Caenorhabditis elegans, we have identified RUVB-2, a AAA(+) ATPase, as a novel repressor of a subset of UPR(ER) genes. We show that degradation of RUVB-2 by CDC-48 enhances expression of ER stress response genes through an XBP1-dependent mechanism. The functional interplay between CDC-48 and RUVB-2 in controlling transcription of select UPR(ER) genes appears conserved in human cells. Together, these results describe a novel role for p97/CDC-48, whereby its role in protein degradation is integrated with its role in regulating expression of ER stress response genes

Mots clés

Domaines

Cancer

Dates et versions

hal-01163728 , version 1 (15-06-2015)

Identifiants

Citer

Esther Marza, Saïd Taouji, Kim Barroso, Anne-Aurélie Raymond, Léo Guignard, et al.. Genome-wide screen identifies a novel p97/CDC-48-dependent pathway regulating ER-stress-induced gene transcription. EMBO Reports, 2015, 16 (3), pp.332--340. ⟨10.15252/embr.201439123⟩. ⟨hal-01163728⟩
280 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More