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Article Dans Une Revue Carbohydrate Research Année : 2015

Araf51 with improved transglycosylation activities: One engineered biocatalyst for one specific acceptor

Résumé

A random mutagenesis of the arabinofuranosyl hydrolase Araf51 has been run in order to access to efficient biocatalysts for the synthesis of alkyl arabinofuranosides. The mutants were selected on their ability to catalyze the transglycosylation reaction of p-nitrophenyl α-L-arabinofuranoside (pNP-Araf) used as a donor and various aliphatic alcohols as acceptors. This screening strategy underlined 5 interesting clones, each one corresponding to one acceptor. They appeared to be much more efficient in the transglycosylation reaction compared to the wild type enzyme whereas no self-condensation or hydrolysis products could be detected. Moreover, the high specificity of the mutants towards the alcohols for which they have been selected validates the screening process. Sequence analysis of the mutated enzymes revealed that, despite their location far from the active site, the mutations affect significantly the kinetics properties as well as the substrate affinity of these mutants towards the alcoholacceptors in the transglycosylation reaction.

Domaines

Chimie organique
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Dates et versions

hal-01086640 , version 1 (24-11-2014)

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Alizé Pennec, Richard Daniellou, Pascal Loyer, Caroline Nugier-Chauvin, Vincent Ferrières. Araf51 with improved transglycosylation activities: One engineered biocatalyst for one specific acceptor. Carbohydrate Research, 2015, 402, pp.50-55. ⟨10.1016/j.carres.2014.10.031⟩. ⟨hal-01086640⟩
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