%0 Journal Article %T Cytoplasmic PAR-3 protein expression is associated with adverse prognostic factors in clear cell renal cell carcinoma and independently impacts survival. %+ Institut de Génétique et Développement de Rennes (IGDR) %+ Service de Cytogénétique et de Biologie Cellulaire %+ Service d'urologie [Rennes] = Urology [Rennes] %+ Service de Pharmacologie [Rennes] %+ Service de génétique médicale %+ Service de néphrologie [Rennes] %A Dagher, Julien %A Dugay, Frédéric %A Rioux-Leclercq, Nathalie %A Verhoest, Gregory %A Oger, Emmanuel %A Bensalah, Karim %A Cabillic, Florian %A Jouan, Florence %A Kammerer-Jacquet, Solene-Florence %A Fergelot, Patricia %A Vigneau, Cécile %A Arlot-Bonnemains, Yannick %A Belaud-Rotureau, Marc-Antoine %Z This work was supported, in part, by grants from the Ligue Contre le Cancer and from the Comite pour la Recherche Clinique et Translationnelle of Rennes University Hospital, Rennes, France. %< avec comité de lecture %@ 0046-8177 %J Human Pathology %I WB Saunders %V 45 %N 8 %P 1639-46 %8 2014-08 %D 2014 %R 10.1016/j.humpath.2014.03.018 %M 24856572 %K PAR-3 protein %K Renal cell carcinoma %K Biomarker %K Prognosis %K Survival %Z Life Sciences [q-bio]/Cellular BiologyJournal articles %X Clear cell renal cell carcinomas (ccRCCs) represent 70% of renal cancers, and several clinical and histolopathological factors are implicated in their prognosis. We recently demonstrated that the overexpression of PAR-3 protein encoded by the PARD3 gene could be implicated in renal oncogenesis. The object of this work was to study the association of intratumoral PAR-3 expression with known prognostic parameters and clinical outcome. In this aim, PAR-3 expression was assessed by immunohistochemistry in ccRCC tumors of 101 patients from 2003 to 2005. The immunostaining of PAR-3 was scored either as membranous (mPAR-3) or as both membranous and cytoplasmic (cPAR-3). Cytoplasmic PAR-3 was significantly associated with worse histopathological and clinical prognostic factors: Fuhrman grades 3 and 4, tumor necrosis, sarcomatoid component, adrenal invasion, renal and hilar fat invasion, eosinophilic component, a noninactivated VHL gene, higher tumor grade, lymph node involvement, metastasis, and worse clinical Eastern Cooperative Oncology Group and S classification scores. After multivariate analysis, 2 parameters were independently associated with cPAR-3: necrosis and eosinophilic components. In addition, cPAR-3 patients had shorter overall and progression-free survivals independently from strong prognostic validated factors like metastases. A cytoplasmic expression of PAR-3 is therefore implicated in worse clinical and pathological cancer features in ccRCC and could be useful to identify patients with high-risk tumors. %G English %2 https://univ-rennes.hal.science/hal-01058621/document %2 https://univ-rennes.hal.science/hal-01058621/file/Cytoplasmic_PAR-3_protein_expression_accepted-1.pdf %L hal-01058621 %U https://univ-rennes.hal.science/hal-01058621 %~ UNIV-RENNES1 %~ CNRS %~ IFR140 %~ HL %~ IGDR %~ STATS-UR1 %~ IGDR-CR %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ UR1-BIO-SA