In vitro development of P- and R-like calcium currents in insect (Periplaneta americana) embryonic brain neurons. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Neuroscience Letters Année : 2004

In vitro development of P- and R-like calcium currents in insect (Periplaneta americana) embryonic brain neurons.

Résumé

Voltage-gated calcium currents are important for the survival and growth of embryonic cockroach brain neurons in primary culture. In the present experiments, we have studied, using the patch-clamp technique, the evolution with time in culture of the voltage-dependency and of the pharmacological properties of the calcium conductance of these neurons during the formation of a network. We have observed a progressive increase of the high-voltage-activated calcium conductance and a 10mV shift of the voltage-dependency of activation towards more negative potentials. The proportion of the R-like calcium current component increased during network formation. At the same time, the highly omega-AgaTxIVA-sensitive P-like component of the current is progressively replaced by a component which is less sensitive to the toxin. The origin and functional implications of these modifications are discussed.

Domaines

Neurosciences

Dates et versions

hal-00982273 , version 1 (23-04-2014)

Identifiants

Citer

Pascal Benquet, Yves Pichon, François Tiaho. In vitro development of P- and R-like calcium currents in insect (Periplaneta americana) embryonic brain neurons.. Neuroscience Letters, 2004, 365 (3), pp.228-32. ⟨10.1016/j.neulet.2004.05.002⟩. ⟨hal-00982273⟩
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