EMMPRIN (CD147) regulation of MMP-9 in bronchial epithelial cells in COPD. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Respirology Année : 2011

EMMPRIN (CD147) regulation of MMP-9 in bronchial epithelial cells in COPD.

Résumé

BACKGROUND AND OBJECTIVE: Extracellular matrix metalloproteinase inducer (EMMPRIN or CD147) induces the production of matrix metalloproteinases (MMP) such as MMP-9, which plays an important role in COPD. We determined its cellular origin and role in MMP-9 production in COPD. METHODS: Bronchial biopsies, alveolar macrophages (AM) and blood monocytes (BM) from patients with COPD, healthy smokers and non-smokers, and bronchial epithelial cells (EC) from surgically resected airways from patients with COPD were stimulated with LPS or CRP in the presence and absence of an anti-EMMPRIN blocking antibody. EMMPRIN in BAL, plasma, conditioned media and cell lysates was quantified and immunohistochemical localization of EMMPRIN was determined in bronchial biopsies. MMP-9 activity and mRNA was also determined. RESULTS: EMMPRIN levels in BAL fluid were higher in patients with COPD compared with non-smokers and smokers. There was greater EMMPRIN expression in EC from patients with COPD compared with smokers and non-smokers. EC secreted and expressed more EMMPRIN protein than BM and AM. Blocking EMMPRIN decreased MMP-9 activity in supernatant of EC, but not in those from AM and BM, and decreased MMP-9 mRNA expression in EC. CONCLUSIONS: The increased EMMPRIN expression in COPD is reflected by an increased release from bronchial EC, which are one of the main source of EMMPRIN. EMMPRIN regulates MMP-9 expression in COPD.

Dates et versions

hal-00873269 , version 1 (15-10-2013)

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Stephane Jouneau, Nadia Khorasani, Patricia de Souza, Patricia Macedo, Jie Zhu, et al.. EMMPRIN (CD147) regulation of MMP-9 in bronchial epithelial cells in COPD.. Respirology, 2011, 16 (4), pp.705-12. ⟨10.1111/j.1440-1843.2011.01960.x⟩. ⟨hal-00873269⟩
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